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Influence of Bone and Muscle Injuries on the Osteogenic Potential of Muscle Progenitors: Contribution of Tissue Environment to Heterotopic Ossification

التفاصيل البيبلوغرافية
العنوان: Influence of Bone and Muscle Injuries on the Osteogenic Potential of Muscle Progenitors: Contribution of Tissue Environment to Heterotopic Ossification
المؤلفون: Jeremy Molligan, Reed Mitchell, Lew Schon, Samuel Achilefu, Talal Zahoor, Young Cho, Jeffery Loube, Zijun Zhang
المصدر: Stem Cells Translational Medicine, Vol 5, Iss 6, Pp 745-753 (2016)
بيانات النشر: Oxford University Press, 2016.
سنة النشر: 2016
المجموعة: LCC:Medicine (General)
LCC:Cytology
مصطلحات موضوعية: Heterotopic ossification, Osteogenesis, Progenitors, Mesenchymal stem cells, Muscle, Medicine (General), R5-920, Cytology, QH573-671
الوصف: By using surgical mouse models, this study investigated how the tissue environment influences the osteogenic potential of muscle progenitors (m‐progenitors) and potentially contributes to heterotopic ossification (HO). Injury was induced by clamping the gluteus maximus and medius (group M) or osteotomy of greater trochanter (group O) on the right hip, as well as combined muscle injury and osteotomy of greater trochanter (group M+O). The gluteus maximus and medius of the operated hips were harvested at days 1, 3, 5, and 10 for isolation of m‐progenitors. The cells were cultured in an osteogenic medium for 3 weeks, and osteogenesis was evaluated by matrix mineralization and the expression of osteogenesis‐related genes. The expression of type I collagen, RUNX2 (runt‐related transcription factor 2), and osteocalcin by the m‐progenitors of group M+O was significantly increased, compared with groups M and O. Osteogenic m‐progenitors in group O increased the expression of bone morphogenetic protein 2 and also bone morphogenetic protein antagonist differential screening‐selected gene aberrative in neuroblastoma. On histology, there was calcium deposition mostly in the muscles of group M+O harvested at day 10. CD56, representing myogenic progenitors, was highly expressed in the m‐progenitors isolated from group M (day 10), but m‐progenitors of group M+O (day 10) exhibited the highest expression of platelet‐derived growth factor receptor α (PDGFR‐α), a marker of muscle‐derived mesenchymal stem cells (M‐MSCs). The expressions of PDGFR‐α and RUNX2 were colocalized in osteogenic m‐progenitors. The data indicate that the tissue environment simulated in the M+O model is a favorable condition for HO formation. Most likely, M‐MSCs, rather than myogenic progenitors, in the m‐progenitors participate in HO formation. Significance The prevalence of traumatic heterotopic ossification (HO) is high in war injury. The pathogenesis of HO is still unknown. This study clarified the contribution of a tissue environment created by bone or muscle injury to the formation of HO. The study also found that muscle‐derived mesenchymal stem cells, but not myogenic progenitors, are involved in the formation of HO. The findings of this study could be used to strategize the prevention and treatment of HO.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2157-6580
2157-6564
Relation: https://doaj.org/toc/2157-6564; https://doaj.org/toc/2157-6580
DOI: 10.5966/sctm.2015-0082
URL الوصول: https://doaj.org/article/5ad0aa289f09414d960c0fe8b1114aae
رقم الأكسشن: edsdoj.5ad0aa289f09414d960c0fe8b1114aae
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:21576580
21576564
DOI:10.5966/sctm.2015-0082