دورية أكاديمية
Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize
| العنوان: | Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize |
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| المؤلفون: | Yuan-Yuan Jiang, Yi-Ping Chai, Min-Hui Lu, Xiu-Li Han, Qiupeng Lin, Yu Zhang, Qiang Zhang, Yun Zhou, Xue-Chen Wang, Caixia Gao, Qi-Jun Chen |
| المصدر: | Genome Biology, Vol 21, Iss 1, Pp 1-10 (2020) |
| بيانات النشر: | BMC, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Biology (General) LCC:Genetics |
| مصطلحات موضوعية: | Prime editing, PE2, pegRNA, CRISPR/Cas9, Maize, ALS, Biology (General), QH301-705.5, Genetics, QH426-470 |
| الوصف: | Abstract Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1474-760X |
| Relation: | http://link.springer.com/article/10.1186/s13059-020-02170-5; https://doaj.org/toc/1474-760X |
| DOI: | 10.1186/s13059-020-02170-5 |
| URL الوصول: | https://doaj.org/article/5ae751de190c46b0a5d55075e84a8549 |
| رقم الأكسشن: | edsdoj.5ae751de190c46b0a5d55075e84a8549 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1474760X |
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| DOI: | 10.1186/s13059-020-02170-5 |