دورية أكاديمية
DNA extraction from formalin-fixed tissue: new light from the deep sea
| العنوان: | DNA extraction from formalin-fixed tissue: new light from the deep sea |
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| المؤلفون: | Ferran Palero, Sally Hall, Paul F. Clark, David Johnston, Jackie Mackenzie-Dodds, Sven Thatje |
| المصدر: | Scientia Marina, Vol 74, Iss 3, Pp 465-470 (2010) |
| بيانات النشر: | Consejo Superior de Investigaciones Científicas, 2010. |
| سنة النشر: | 2010 |
| المجموعة: | LCC:Aquaculture. Fisheries. Angling |
| مصطلحات موضوعية: | tetramethylsilane, ethidium bromide, formalin, carcinus, lithodidae, Aquaculture. Fisheries. Angling, SH1-691 |
| الوصف: | DNA samples were extracted from ethanol and formalin-fixed decapod crustacean tissue using a new method based on Tetramethylsilane (TMS)-Chelex. It is shown that neither an indigestible matrix of cross-linked protein nor soluble PCR inhibitors impede PCR success when dealing with formalin-fixed material. Instead, amplification success from formalin-fixed tissue appears to depend on the presence of unmodified DNA in the extracted sample. A staining method that facilitates the targeting of samples with a high content of unmodified DNA is provided. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0214-8358 1886-8134 |
| Relation: | http://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/1175; https://doaj.org/toc/0214-8358; https://doaj.org/toc/1886-8134 |
| DOI: | 10.3989/scimar.2010.74n3465 |
| URL الوصول: | https://doaj.org/article/5b0b20a9faf548258f06e224ec53a613 |
| رقم الأكسشن: | edsdoj.5b0b20a9faf548258f06e224ec53a613 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 02148358 18868134 |
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| DOI: | 10.3989/scimar.2010.74n3465 |