دورية أكاديمية
Purification and selected biochemical properties of peroxidase from cress (Lepidium sativum sub sp. sativum)
| العنوان: | Purification and selected biochemical properties of peroxidase from cress (Lepidium sativum sub sp. sativum) |
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| المؤلفون: | Ahmet Altay, Tubanur Koktepe, Lokman Durmaz, Fevzi Topal, İlhami Gülçin, Ekrem Köksal |
| المصدر: | International Journal of Food Properties, Vol 21, Iss 1, Pp 2610-2621 (2018) |
| بيانات النشر: | Taylor & Francis Group, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Nutrition. Foods and food supply LCC:Food processing and manufacture |
| مصطلحات موضوعية: | Cress (Lepidium sativum), peroxidase, enzyme purification, enzyme characterization, enzyme inhabitation, Nutrition. Foods and food supply, TX341-641, Food processing and manufacture, TP368-456 |
| الوصف: | Cress (Lepidium sativum) is an annual herb from Brassicaceae family, and in some regions. It is known as peppergrass, garden cress, garden peppercress, pepperwort, or poor man’s pepper. Cress is an important medicinal plant in some countries including Turkey. This plant has major scientific and therapeutic significance. Peroxidase (POD, E.C.1.11.1.7) is an oxidoreductase enzyme produced by a number of organisms. In this study, POD enzyme was purified from cress by ammonium sulphate precipitation, gel filtration, and CM-Sephadex ion-exchange chromatographies. Km and Vmax values were calculated from the Lineweaver-Burk graph for H2O2 and guaiacol substrates and the substrate specificity of POD was investigated. The results showed that substrate specificity of H2O2 is better than substrate specificity of guaiacol for this enzyme. The inhibition effects of three cations (Al3+, Cu2+, and Cr3+) and one organic compound of cetyl trimethylammonium bromide were performed and their inhibition types were determined. Finally, optimum pH, optimum temperature, optimum ionic strength, and stable pH were determined. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1094-2912 1532-2386 10942912 |
| Relation: | https://doaj.org/toc/1094-2912; https://doaj.org/toc/1532-2386 |
| DOI: | 10.1080/10942912.2018.1540989 |
| URL الوصول: | https://doaj.org/article/5bd260df989b44b19e587f6b7bf85adc |
| رقم الأكسشن: | edsdoj.5bd260df989b44b19e587f6b7bf85adc |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 10942912 15322386 |
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| DOI: | 10.1080/10942912.2018.1540989 |