دورية أكاديمية
Adult-Brain-Derived Neural Stem Cells Grafting into a Vein Bridge Increases Postlesional Recovery and Regeneration in a Peripheral Nerve of Adult Pig
| العنوان: | Adult-Brain-Derived Neural Stem Cells Grafting into a Vein Bridge Increases Postlesional Recovery and Regeneration in a Peripheral Nerve of Adult Pig |
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| المؤلفون: | Olivier Liard, Stéphanie Segura, Emmanuel Sagui, André Nau, Aurélie Pascual, Melissa Cambon, Jean-Luc Darlix, Thierry Fusai, Emmanuel Moyse |
| المصدر: | Stem Cells International, Vol 2012 (2012) |
| بيانات النشر: | Wiley, 2012. |
| سنة النشر: | 2012 |
| المجموعة: | LCC:Internal medicine |
| مصطلحات موضوعية: | Internal medicine, RC31-1245 |
| الوصف: | We attempted transplantation of adult neural stem cells (ANSCs) inside an autologous venous graft following surgical transsection of nervis cruralis with 30 mm long gap in adult pig. The transplanted cell suspension was a primary culture of neurospheres from adult pig subventricular zone (SVZ) which had been labeled in vitro with BrdU or lentivirally transferred fluorescent protein. Lesion-induced loss of leg extension on the thigh became definitive in controls but was reversed by 45–90 days after neurosphere-filled vein grafting. Electromyography showed stimulodetection recovery in neurosphere-transplanted pigs but not in controls. Postmortem immunohistochemistry revealed neurosphere-derived cells that survived inside the venous graft from 10 to 240 post-lesion days and all displayed a neuronal phenotype. Newly formed neurons were distributed inside the venous graft along the severed nerve longitudinal axis. Moreover, ANSC transplantation increased CNPase expression, indicating activation of intrinsic Schwann cells. Thus ANSC transplantation inside an autologous venous graft provides an efficient repair strategy. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1687-966X 1687-9678 |
| Relation: | https://doaj.org/toc/1687-966X; https://doaj.org/toc/1687-9678 |
| DOI: | 10.1155/2012/128732 |
| URL الوصول: | https://doaj.org/article/5bd35f80a1f84a7684a1a674d29e6395 |
| رقم الأكسشن: | edsdoj.5bd35f80a1f84a7684a1a674d29e6395 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1687966X 16879678 |
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| DOI: | 10.1155/2012/128732 |