دورية أكاديمية
Targeting Oxidative Stress Injury after Ischemic Stroke in Conscious Rats: Limited Benefits with Apocynin Highlight the Need to Incorporate Long Term Recovery
| العنوان: | Targeting Oxidative Stress Injury after Ischemic Stroke in Conscious Rats: Limited Benefits with Apocynin Highlight the Need to Incorporate Long Term Recovery |
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| المؤلفون: | Robert M. Weston, Bin Lin, Gregory J. Dusting, Carli L. Roulston |
| المصدر: | Stroke Research and Treatment, Vol 2013 (2013) |
| بيانات النشر: | Wiley, 2013. |
| سنة النشر: | 2013 |
| المجموعة: | LCC:Neurology. Diseases of the nervous system |
| مصطلحات موضوعية: | Neurology. Diseases of the nervous system, RC346-429 |
| الوصف: | NADPH oxidase is a major source of superoxide anion following stroke and reperfusion. This study evaluated the effects of apocynin, a known antioxidant and inhibitor of Nox2 NADPH, on neuronal injury and cell-specific responses to stroke induced in the conscious rat. Apocynin treatment (50 mg/kg i.p.) commencing 1 hour prior to stroke and 24 and 48 hours after stroke significantly reduced infarct volume in the cortex by ~ 60%, but had no effect on striatal damage or neurological deficits. In situ detection of reactive oxygen species (ROS) using dihydroethidium fluorescence revealed that increased ROS detected in OX-42 positive cells following ischemia was reduced in apocynin-treated rats by ~ 51%, but surprisingly increased in surrounding NeuN positive cells of the same rats by ~ 27%, in comparison to the contralateral hemisphere. Reduced ROS from activated microglia/macrophages treated with apocynin was associated with reduced Nox2 immunoreactivity without change to the number of cells. These findings confirm the protective effects of apocynin and indicate a novel mechanism via reduced Nox2 expression. We also reveal compensatory changes in neuronal ROS generation as a result of Nox2 inhibition and highlight the need to assess long term individual cell responses to inhibitors of oxidative stress. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2090-8105 2042-0056 |
| Relation: | https://doaj.org/toc/2090-8105; https://doaj.org/toc/2042-0056 |
| DOI: | 10.1155/2013/648061 |
| URL الوصول: | https://doaj.org/article/5cfe927aaad1496d985858ecd1171cea |
| رقم الأكسشن: | edsdoj.5cfe927aaad1496d985858ecd1171cea |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20908105 20420056 |
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| DOI: | 10.1155/2013/648061 |