دورية أكاديمية
Characterization of neurotropic HPAI H5N1 viruses with novel genome constellations and mammalian adaptive mutations in free-living mesocarnivores in Canada
| العنوان: | Characterization of neurotropic HPAI H5N1 viruses with novel genome constellations and mammalian adaptive mutations in free-living mesocarnivores in Canada |
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| المؤلفون: | Tamiru N. Alkie, Sherri Cox, Carissa Embury-Hyatt, Brian Stevens, Neil Pople, Margo J. Pybus, Wanhong Xu, Tamiko Hisanaga, Matthew Suderman, Janice Koziuk, Peter Kruczkiewicz, Hoang Hai Nguyen, Mathew Fisher, Oliver Lung, Cassidy N. G. Erdelyan, Orie Hochman, Davor Ojkic, Carmencita Yason, Maria Bravo-Araya, Laura Bourque, Trent K. Bollinger, Catherine Soos, Jolene Giacinti, Jennifer Provencher, Sarah Ogilvie, Amanda Clark, Robyn MacPhee, Glen J. Parsons, Hazel Eaglesome, Sayrah Gilbert, Kelsey Saboraki, Richard Davis, Alexandra Jerao, Matthew Ginn, Megan E.B. Jones, Yohannes Berhane |
| المصدر: | Emerging Microbes and Infections, Vol 12, Iss 1 (2023) |
| بيانات النشر: | Taylor & Francis Group, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Infectious and parasitic diseases LCC:Microbiology |
| مصطلحات موضوعية: | Clade 2.3.4.4b, HPAI, H5N1, reassortment, mutation, mammals, Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502 |
| الوصف: | ABSTRACTThe GsGd lineage (A/goose/Guangdong/1/1996) H5N1 virus was introduced to Canada in 2021/2022 through the Atlantic and East Asia-Australasia/Pacific flyways by migratory birds. This was followed by unprecedented outbreaks affecting domestic and wild birds, with spillover into other animals. Here, we report sporadic cases of H5N1 in 40 free-living mesocarnivore species such as red foxes, striped skunks, and mink in Canada. The clinical presentations of the disease in mesocarnivores were consistent with central nervous system infection. This was supported by the presence of microscopic lesions and the presence of abundant IAV antigen by immunohistochemistry. Some red foxes that survived clinical infection developed anti-H5N1 antibodies. Phylogenetically, the H5N1 viruses from the mesocarnivore species belonged to clade 2.3.4.4b and had four different genome constellation patterns. The first group of viruses had wholly Eurasian (EA) genome segments. The other three groups were reassortant viruses containing genome segments derived from both North American (NAm) and EA influenza A viruses. Almost 17 percent of the H5N1 viruses had mammalian adaptive mutations (E627 K, E627V and D701N) in the polymerase basic protein 2 (PB2) subunit of the RNA polymerase complex. Other mutations that may favour adaptation to mammalian hosts were also present in other internal gene segments. The detection of these critical mutations in a large number of mammals within short duration after virus introduction inevitably highlights the need for continually monitoring and assessing mammalian-origin H5N1 clade 2.3.4.4b viruses for adaptive mutations, which potentially can facilitate virus replication, horizontal transmission and posing pandemic risks for humans. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 22221751 2222-1751 |
| Relation: | https://doaj.org/toc/2222-1751 |
| DOI: | 10.1080/22221751.2023.2186608 |
| URL الوصول: | https://doaj.org/article/5d9cd1d630384b6cb49ee8d782120ce7 |
| رقم الأكسشن: | edsdoj.5d9cd1d630384b6cb49ee8d782120ce7 |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 22221751 |
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| DOI: | 10.1080/22221751.2023.2186608 |