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Methods for Engineering Binders to Multi-Pass Membrane Proteins

التفاصيل البيبلوغرافية
العنوان: Methods for Engineering Binders to Multi-Pass Membrane Proteins
المؤلفون: Benjamin Thomas, Karuppiah Chockalingam, Zhilei Chen
المصدر: Bioengineering, Vol 10, Iss 12, p 1351 (2023)
بيانات النشر: MDPI AG, 2023.
سنة النشر: 2023
المجموعة: LCC:Technology
LCC:Biology (General)
مصطلحات موضوعية: transmembrane proteins, multi-pass transmembrane proteins, nanodisc, SMALP, panning, directed evolution, Technology, Biology (General), QH301-705.5
الوصف: Numerous potential drug targets, including G-protein-coupled receptors and ion channel proteins, reside on the cell surface as multi-pass membrane proteins. Unfortunately, despite advances in engineering technologies, engineering biologics against multi-pass membrane proteins remains a formidable task. In this review, we focus on the different methods used to prepare/present multi-pass transmembrane proteins for engineering target-specific biologics such as antibodies, nanobodies and synthetic scaffold proteins. The engineered biologics exhibit high specificity and affinity, and have broad applications as therapeutics, probes for cell staining and chaperones for promoting protein crystallization. We primarily cover publications on this topic from the past 10 years, with a focus on the different formats of multi-pass transmembrane proteins. Finally, the remaining challenges facing this field and new technologies developed to overcome a number of obstacles are discussed.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2306-5354
Relation: https://www.mdpi.com/2306-5354/10/12/1351; https://doaj.org/toc/2306-5354
DOI: 10.3390/bioengineering10121351
URL الوصول: https://doaj.org/article/aa5d9e78b6314eb3abca7811927c5f74
رقم الأكسشن: edsdoj.5d9e78b6314eb3abca7811927c5f74
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:23065354
DOI:10.3390/bioengineering10121351