دورية أكاديمية
Methods for Engineering Binders to Multi-Pass Membrane Proteins
العنوان: | Methods for Engineering Binders to Multi-Pass Membrane Proteins |
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المؤلفون: | Benjamin Thomas, Karuppiah Chockalingam, Zhilei Chen |
المصدر: | Bioengineering, Vol 10, Iss 12, p 1351 (2023) |
بيانات النشر: | MDPI AG, 2023. |
سنة النشر: | 2023 |
المجموعة: | LCC:Technology LCC:Biology (General) |
مصطلحات موضوعية: | transmembrane proteins, multi-pass transmembrane proteins, nanodisc, SMALP, panning, directed evolution, Technology, Biology (General), QH301-705.5 |
الوصف: | Numerous potential drug targets, including G-protein-coupled receptors and ion channel proteins, reside on the cell surface as multi-pass membrane proteins. Unfortunately, despite advances in engineering technologies, engineering biologics against multi-pass membrane proteins remains a formidable task. In this review, we focus on the different methods used to prepare/present multi-pass transmembrane proteins for engineering target-specific biologics such as antibodies, nanobodies and synthetic scaffold proteins. The engineered biologics exhibit high specificity and affinity, and have broad applications as therapeutics, probes for cell staining and chaperones for promoting protein crystallization. We primarily cover publications on this topic from the past 10 years, with a focus on the different formats of multi-pass transmembrane proteins. Finally, the remaining challenges facing this field and new technologies developed to overcome a number of obstacles are discussed. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2306-5354 |
Relation: | https://www.mdpi.com/2306-5354/10/12/1351; https://doaj.org/toc/2306-5354 |
DOI: | 10.3390/bioengineering10121351 |
URL الوصول: | https://doaj.org/article/aa5d9e78b6314eb3abca7811927c5f74 |
رقم الأكسشن: | edsdoj.5d9e78b6314eb3abca7811927c5f74 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 23065354 |
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DOI: | 10.3390/bioengineering10121351 |