دورية أكاديمية
Immobilized collagen prevents shedding and induces sustained GPVI clustering and signaling in platelets
| العنوان: | Immobilized collagen prevents shedding and induces sustained GPVI clustering and signaling in platelets |
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| المؤلفون: | Chiara Pallini, Jeremy A. Pike, Christopher O’Shea, Robert K. Andrews, Elizabeth E. Gardiner, Steve P. Watson, Natalie S. Poulter |
| المصدر: | Platelets, Vol 32, Iss 1, Pp 59-73 (2021) |
| بيانات النشر: | Taylor & Francis Group, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Diseases of the blood and blood-forming organs |
| مصطلحات موضوعية: | glycoprotein (gp) vi, platelets, receptor shedding, signaling, super resolution microscopy, Diseases of the blood and blood-forming organs, RC633-647.5 |
| الوصف: | Collagen, the most thrombogenic constituent of blood vessel walls, activates platelets through glycoprotein VI (GPVI). In suspension, following platelet activation by collagen, GPVI is cleaved by A Disintegrin And Metalloproteinase (ADAM)10 and ADAM17. In this study, we use single-molecule localization microscopy and a 2-level DBSCAN-based clustering tool to show that GPVI remains clustered along immobilized collagen fibers for at least 3 hours in the absence of significant shedding. Tyrosine phosphorylation of spleen tyrosine kinase (Syk) and Linker of Activated T cells (LAT), and elevation of intracellular Ca2+, are sustained over this period. Syk, but not Src kinase-dependent signaling is required to maintain clustering of the collagen integrin α2β1, whilst neither is required for GPVI. We propose that clustering of GPVI on immobilized collagen protects GPVI from shedding in order to maintain sustained Src and Syk-kinases dependent signaling, activation of integrin α2β1, and continued adhesion. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0953-7104 1369-1635 09537104 |
| Relation: | https://doaj.org/toc/0953-7104; https://doaj.org/toc/1369-1635 |
| DOI: | 10.1080/09537104.2020.1849607 |
| URL الوصول: | https://doaj.org/article/5e7b1596d96d4c4aadc66a0f0873e18a |
| رقم الأكسشن: | edsdoj.5e7b1596d96d4c4aadc66a0f0873e18a |
| قاعدة البيانات: | Directory of Open Access Journals |
PDF full text from Publisher | تدمد: | 09537104 13691635 |
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| DOI: | 10.1080/09537104.2020.1849607 |