دورية أكاديمية
Diosmin Enhances the Anti-migration Activity of Curcumin Analog PGV-1 on Colorectal Cancer Cells
| العنوان: | Diosmin Enhances the Anti-migration Activity of Curcumin Analog PGV-1 on Colorectal Cancer Cells |
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| المؤلفون: | Muthi Ikawati, Rohmad Yudi Utomo, Novia Permata Hapsari, Edy Meiyanto, Chio Oka |
| المصدر: | Indonesian Biomedical Journal, Vol 16, Iss 1, Pp 56-65 (2024) |
| بيانات النشر: | Secretariat of The Indonesian Biomedical Journal, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Medicine (General) |
| مصطلحات موضوعية: | Medicine (General), R5-920 |
| الوصف: | BACKGROUND: Diosmin enhances the cytotoxicity of Pentagamavunone-1 (PGV-1) in cancer cells. PGV-1 and diosmin are predicted to target several matrix metalloproteinases (MMPs) in metastatic cancer, including colorectal cancer, but the anti-migration potency of their combination has not established yet. This study evaluates the anti-migration effect of PGV-1 and diosmin combination in colorectal cancer. METHODS: The cytotoxicity assay using Cell Counting Kit 8 (CCK-8) method in WiDr colorectal cancer cells was carried out to determine the concentration for anti-migration experiments. The wound healing assay was used to observe the anti-migration activity by measuring the cell-free area. Gelatin zymography was employed to detect the MMP activity indicating by the clear band density. The interaction between PGV-1 or diosmin and MMP proteins was predicted by molecular dockings. RESULTS: PGV-1 was cytotoxic (IC50 17 mM), while diosmin up to 100 mM did not affect cell viability. Both 10 mM PGV-1 as well as 50 and 100 mM diosmin slowed down the closure of cell-free area. A 100 mM diosmin was significantly enhance the anti-migratory activity of 50 and 100 mM PGV-1. The activity of MMP-9 and MMP-2 was also lower in the presence of diosmin compared to than that of PGV-1 alone. PGV-1 or diosmin was also able to interact with MMP proteins with a lower energy compared to than that of the native ligands. CONCLUSION: Diosmin enhances the anti-migration activity of PGV-1 in WiDr cells, possibly by affecting MMPs’ activity. This study is an evidence that diosmin is a potential co-chemotherapy candidate for PGV-1, that can be utilized to overcome metastatis in colorectal cancer. KEYWORDS: cancer, citrus flavonoid, co-chemotherapy, diosmin, matrix metalloproteinases (MMPs), migration, Pentagamavunone-1, WiDr cancer cell |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2085-3297 2355-9179 |
| Relation: | https://inabj.org/index.php/ibj/article/view/2829; https://doaj.org/toc/2085-3297; https://doaj.org/toc/2355-9179 |
| DOI: | 10.18585/inabj.v16i1.2829 |
| URL الوصول: | https://doaj.org/article/e5eb4a70bada4b16836b53d5d0e65905 |
| رقم الأكسشن: | edsdoj.5eb4a70bada4b16836b53d5d0e65905 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20853297 23559179 |
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| DOI: | 10.18585/inabj.v16i1.2829 |