دورية أكاديمية
Ovarian Cell Encapsulation in an Enzymatically Crosslinked Silk-Based Hydrogel with Tunable Mechanical Properties
| العنوان: | Ovarian Cell Encapsulation in an Enzymatically Crosslinked Silk-Based Hydrogel with Tunable Mechanical Properties |
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| المؤلفون: | Hafez Jafari, Arezoo Dadashzadeh, Saeid Moghassemi, Payam Zahedi, Christiani A. Amorim, Amin Shavandi |
| المصدر: | Gels, Vol 7, Iss 3, p 138 (2021) |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Science LCC:Chemistry LCC:Inorganic chemistry LCC:General. Including alchemy |
| مصطلحات موضوعية: | ovarian stromal cells encapsulation, injectable hydrogel, enzymatic crosslinking, artificial ovary, silk fibroin, Science, Chemistry, QD1-999, Inorganic chemistry, QD146-197, General. Including alchemy, QD1-65 |
| الوصف: | An artificial ovary is a promising approach for preserving fertility in prepubertal girls and women who cannot undergo current cryopreservation strategies. However, this approach is in its infancy, due to the possible challenges of creating a suitable 3D matrix for encapsulating ovarian follicles and stromal cells. To maintain the ovarian stromal cell viability and proliferation, as a first step towards developing an artificial ovary, in this study, a double network hydrogel with a high water swelling capacity (swelling index 15–19) was developed, based on phenol conjugated chitosan (Cs-Ph) and silk fibroin (SF) through an enzymatic crosslinking method using horseradish peroxidase. The addition of SF (1%) to Cs (1%) decreased the storage modulus (G’) from 3500 Pa (Cs1) to 1600 Pa (Cs-SF1), and the hydrogels with a rapid gelation kinetic produced a spatially homogeneous distribution of ovarian cells that demonstrated 167% proliferation after 7 days. This new Cs-SF hydrogel benefits from the toughness and flexibility of SF, and phenolic chemistry could provide the potential microstructure for encapsulating human ovarian stromal cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2310-2861 |
| Relation: | https://www.mdpi.com/2310-2861/7/3/138; https://doaj.org/toc/2310-2861 |
| DOI: | 10.3390/gels7030138 |
| URL الوصول: | https://doaj.org/article/e5ed192700574e4fb278e5ccfe09c2b1 |
| رقم الأكسشن: | edsdoj.5ed192700574e4fb278e5ccfe09c2b1 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23102861 |
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| DOI: | 10.3390/gels7030138 |