دورية أكاديمية
Association of enzymatic and optimized ultrasound-assisted aqueous extraction of flavonoid glycosides from dried Hippophae rhamnoides L. (Sea Buckthorn) berries
| العنوان: | Association of enzymatic and optimized ultrasound-assisted aqueous extraction of flavonoid glycosides from dried Hippophae rhamnoides L. (Sea Buckthorn) berries |
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| المؤلفون: | Alexandru Nicolescu, Mihai Babotă, Eduardo Aranda Cañada, Maria Inês Dias, Mikel Añibarro-Ortega, Mihaiela Cornea-Cipcigan, Corneliu Tanase, Cristian Radu Sisea, Andrei Mocan, Lillian Barros, Gianina Crișan |
| المصدر: | Ultrasonics Sonochemistry, Vol 108, Iss , Pp 106955- (2024) |
| بيانات النشر: | Elsevier, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Chemistry LCC:Acoustics. Sound |
| مصطلحات موضوعية: | H. rhamnoides, UAE, EAE, Optimization, Isorhamnetin, Flavonols, Chemistry, QD1-999, Acoustics. Sound, QC221-246 |
| الوصف: | The main purpose of the present study was to determine the effect of associating an optimized ultrasound-assisted extraction (UAE) protocol with enzyme-assisted extraction (EAE) in aqueous media, using the dried berries of Hippophae rhamnoides L. (sea buckthorn) as plant material. A specialized software was used for the determination of potential optimal extraction parameters, leading to the development of four optimized extracts with different characteristics (UAE ± EAE). For these extracts, buffered or non-buffered solutions have been used, with the aim to determine the influence of adjustable pH on extractability. As enzymatic solution, a pectinase, cellulase, and hemicellulase mix (2:1:1) has been applied, acting as pre-treatment for the optimized protocol. The highest extractive yields have been identified for non-buffered extracts, and the E-UAE combination obtained extracts with the highest overall in vitro antioxidant activity. The HPLC-MSn analysis demonstrated a rich composition in different types of isorhamnetin-O-glycosides, as well as some quercetin-O-glycosides, showing a high recovery of specific flavonol-type polyphenolic species. Moreover, we have tentatively identified two flavanols (i.e., catechin and epigallocatechin) and one flavone derivative (i.e., luteolin). |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1350-4177 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S1350417724002037; https://doaj.org/toc/1350-4177 |
| DOI: | 10.1016/j.ultsonch.2024.106955 |
| URL الوصول: | https://doaj.org/article/ae5f1e5c187d409b876d7f91ddf54051 |
| رقم الأكسشن: | edsdoj.5f1e5c187d409b876d7f91ddf54051 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 13504177 |
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| DOI: | 10.1016/j.ultsonch.2024.106955 |