دورية أكاديمية
Circular RNA hsa_circ_0007444 inhibits ovarian cancer progression through miR-23a-3p/DICER1 axis
| العنوان: | Circular RNA hsa_circ_0007444 inhibits ovarian cancer progression through miR-23a-3p/DICER1 axis |
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| المؤلفون: | Zhang Min, Sun Yu, Xu Hanzi, Shi Yaqian, Shen Rong, Teng Fang, Xu Juan, Jia Xuemei |
| المصدر: | Acta Biochimica et Biophysica Sinica, Vol 55, Pp 574-586 (2023) |
| بيانات النشر: | China Science Publishing & Media Ltd., 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biochemistry LCC:Genetics |
| مصطلحات موضوعية: | ovarian cancer, hsa_circ_0007444, miR-23a-3p, DICER1, progression, Biochemistry, QD415-436, Genetics, QH426-470 |
| الوصف: | Ovarian cancer is the second leading cause of death in women with gynecological malignancy in China. Circular RNAs are a class of noncoding regulatory RNAs reported to be involved in cancer development and progression. Previous studies, including our own, have indicated that hsa_circ_0007444 is downregulated in ovarian cancer tissues. This study aims to elucidate the function and mechanism of hsa_circ_0007444 in ovarian cancer progression. The expression of hsa_circ_0007444 is determined by quantitative real-time PCR (qRT-PCR). Cell proliferation, invasion, migration and apoptosis are examined by cell counting-kit 8 (CCK-8), transwell and flow cytometry assays. Tumor growth and metastasis are assessed in vivo using Balb/c nude mouse xenograft model and tail vein injection model. And the mechanism of action of hsa_circ_0007444 is analysed by RNA-binding protein immunoprecipitation (RIP), luciferase reporter and rescue assays. hsa_circ_0007444 is downregulated in ovarian cancer tissues and cell lines compared with that in normal ovarian tissues and normal epithelial cell line. Gain- and loss-of-function results indicate that hsa_circ_0007444 inhibits cell proliferation, invasion, migration and increases cell apoptosis of ovarian cancer cells in vitro, and inhibits tumor growth and lung metastasis in vivo. Mechanistically, hsa_circ_0007444 can interact with AGO2 and sponge miR-23a-3p, thereby upregulating DICER1 expression, which is an important tumor suppressor in ovarian cancer. And miR-23a-3p mimics can rescue the inhibitory effect of hsa_circ_0007444 on ovarian cancer cell proliferation, invasion and migration. Therefore, hsa_circ_0007444 can inhibit ovarian cancer progression through the hsa_circ_0007444/miR-23a-3p/DICER1 axis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1672-9145 |
| Relation: | https://doaj.org/toc/1672-9145 |
| DOI: | 10.3724/abbs.2023052 |
| URL الوصول: | https://doaj.org/article/5f2d301019464e4a95adf219f934330a |
| رقم الأكسشن: | edsdoj.5f2d301019464e4a95adf219f934330a |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16729145 |
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| DOI: | 10.3724/abbs.2023052 |