دورية أكاديمية
Integration of Whole-Genome Resequencing and Transcriptome Sequencing Reveals Candidate Genes in High Glossiness of Eggshell
| العنوان: | Integration of Whole-Genome Resequencing and Transcriptome Sequencing Reveals Candidate Genes in High Glossiness of Eggshell |
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| المؤلفون: | Xiang Song, Shuo Li, Shixiong He, Hongxiang Zheng, Ruijie Li, Long Liu, Tuoyu Geng, Minmeng Zhao, Daoqing Gong |
| المصدر: | Animals, Vol 14, Iss 8, p 1141 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Veterinary medicine LCC:Zoology |
| مصطلحات موضوعية: | whole-genome resequencing, RNA-seq, eggshell gloss, Veterinary medicine, SF600-1100, Zoology, QL1-991 |
| الوصف: | Eggshell gloss is an important characteristic for the manifestation of eggshell appearance. However, no study has yet identified potential candidate genes for eggshell gloss between high-gloss (HG) and low-gloss (LG) chickens. The aim of this study was to perform a preliminary investigation into the formation mechanism of eggshell gloss and to identify potential genes. The eggshell gloss of 300-day-old Rhode Island Red hens was measured from three aspects. Uterine tissues of the selected HG and LG (n = 5) hens were collected for RNA-seq. Blood samples were also collected for whole-genome resequencing (WGRS). RNA-seq analysis showed that 150 differentially expressed genes (DEGs) were identified in the uterine tissues of HG and LG hens. These DEGs were mainly enriched in the calcium signaling pathway and the neuroactive ligand–receptor interaction pathway. Importantly, these two pathways were also significantly enriched in the WGRS analysis results. Further joint analysis of WGRS and RNA-seq data revealed that 5-hydroxytryptamine receptor 1F (HTR1F), zinc finger protein 536 (ZNF536), NEDD8 ubiquitin-like modifier (NEDD8), nerve growth factor (NGF) and calmodulin 1 (CALM1) are potential candidate genes for eggshell gloss. In summary, our research provides a reference for the study of eggshell gloss and lays a foundation for improving egg glossiness in layer breeding. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2076-2615 |
| Relation: | https://www.mdpi.com/2076-2615/14/8/1141; https://doaj.org/toc/2076-2615 |
| DOI: | 10.3390/ani14081141 |
| URL الوصول: | https://doaj.org/article/601b452eaec045f58dbb23127e6b6440 |
| رقم الأكسشن: | edsdoj.601b452eaec045f58dbb23127e6b6440 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20762615 |
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| DOI: | 10.3390/ani14081141 |