دورية أكاديمية
Automated measurement of transepithelial electrical resistance (TEER) in 96-well transwells using ECIS TEER96: Single and multiple time point assessments
| العنوان: | Automated measurement of transepithelial electrical resistance (TEER) in 96-well transwells using ECIS TEER96: Single and multiple time point assessments |
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| المؤلفون: | Jacob Schimetz, Pranav Shah, Charles Keese, Chris Dehnert, Michael Detweiler, Sam Michael, Catherine Toniatti-Yanulavich, Xin Xu, Elias C. Padilha |
| المصدر: | SLAS Technology, Vol 29, Iss 1, Pp 100116- (2024) |
| بيانات النشر: | Elsevier, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Biotechnology LCC:Medical technology |
| مصطلحات موضوعية: | Transtepithelial electrical resistance (TEER), Caco-2, MDCK, Membrane biology, Automation, Biotechnology, TP248.13-248.65, Medical technology, R855-855.5 |
| الوصف: | Transepithelial electrical resistance (TEER) is a widely used technique for quantifying the permeability of epithelial and endothelial cell layers. However, traditional methods of measuring TEER are limited to single timepoint measurements and can subject cells to an altered environment during the measurement. Here, we assessed the validity of TEER measurements by the ECIS TEER96 device, which is designed to take continuous TEER measurements of a cell culture system in a standard laboratory incubator. We found that the instrument accurately measures TEER across TEER values ranging from 10 to 2050 Ω*cm2 and is more accurate than the manual epithelial voltohmmeter electrode at high TEER values. Furthermore, the high-resolution measurements provided by the device allowed for a unique insight into the mechanisms and kinetics of cells in vitro. To demonstrate the continuous measurement capability of the device, we tracked the formation of an MDCKI cell monolayer until TEER plateaued. Furthermore, we treated Caco-2 monolayers with different concentrations of DMSO and the antimicrobial and surfactant compound benzethonium chloride to measure disruptions to barrier integrity. Treatment of both compounds resulted in concentration-dependent loss of barrier integrity. Our results suggest that the ECIS TEER96 device is a reliable and convenient option for measuring TEER in cell cultures and can provide valuable insights into the behavior of cells in vitro. This technology will be especially useful for increasing throughput of drug permeability assays, inflammation studies, and gaining better understanding of disease states in a cell culture system. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2472-6303 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2472630323000651; https://doaj.org/toc/2472-6303 |
| DOI: | 10.1016/j.slast.2023.10.008 |
| URL الوصول: | https://doaj.org/article/a609b454386141629841489eac1b65f4 |
| رقم الأكسشن: | edsdoj.609b454386141629841489eac1b65f4 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24726303 |
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| DOI: | 10.1016/j.slast.2023.10.008 |