دورية أكاديمية

Mannose-Functionalized Isoniazid-Loaded Nanostructured Lipid Carriers for Pulmonary Delivery: In Vitro Prospects and In Vivo Therapeutic Efficacy Assessment

التفاصيل البيبلوغرافية
العنوان: Mannose-Functionalized Isoniazid-Loaded Nanostructured Lipid Carriers for Pulmonary Delivery: In Vitro Prospects and In Vivo Therapeutic Efficacy Assessment
المؤلفون: Shaveta Ahalwat, Dinesh Chandra Bhatt, Surbhi Rohilla, Vikas Jogpal, Kirti Sharma, Tarun Virmani, Girish Kumar, Abdulsalam Alhalmi, Ali S. Alqahtani, Omar M. Noman, Marwan Almoiliqy
المصدر: Pharmaceuticals, Vol 16, Iss 8, p 1108 (2023)
بيانات النشر: MDPI AG, 2023.
سنة النشر: 2023
المجموعة: LCC:Medicine
LCC:Pharmacy and materia medica
مصطلحات موضوعية: isoniazid, nanostructured lipid carriers, in vivo pharmacokinetics, drug release profile, histopathological toxicity, mannosylation, Medicine, Pharmacy and materia medica, RS1-441
الوصف: Resistance to isoniazid (INH) is common and increases the possibility of acquiring multidrug-resistant tuberculosis. For this study, isoniazid-loaded nanostructured lipid carriers (INH-NLCs) were developed and effectively functionalized with mannose (Man) to enhance the residence time of the drug within the lungs via specific delivery and increase the therapeutic efficacy of the formulation. The mannose-functionalized isoniazid-loaded nanostructured lipid carrier (Man-INH-NLC) formulation was evaluated with respect to various formulation parameters, namely, encapsulation efficiency (EE), drug loading (DL), average particle size (PS), zeta potential (ZP), polydispersity index (PDI), in vitro drug release (DR), and release kinetics. The in vitro inhalation behavior of the developed formulation after nebulization was investigated using an Andersen cascade impactor via the estimation of the mass median aerosolized diameter (MMAD) and geometric aerodynamic diameter (GAD) and subsequently found to be suitable for effective lung delivery. An in vivo pharmacokinetic study was carried out in a guinea pig animal model, and it was demonstrated that Man-INH-NLC has a longer residence time in the lungs with improved pharmacokinetics when compared with unfunctionalized INH-NLC, indicating the enhanced therapeutic efficacy of the Man-INH-NLC formulation. Histopathological analysis led us to determine that the extent of tissue damage was more severe in the case of the pure drug solution of isoniazid compared to the Man-INH-NLC formulation after nebulization. Thus, the nebulization of Man-INH-NLC was found to be safe, forming a sound basis for enhancing the therapeutic efficacy of the drug for improved management in the treatment of pulmonary tuberculosis.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1424-8247
Relation: https://www.mdpi.com/1424-8247/16/8/1108; https://doaj.org/toc/1424-8247
DOI: 10.3390/ph16081108
URL الوصول: https://doaj.org/article/60f0af5f22054050918f429d6992469a
رقم الأكسشن: edsdoj.60f0af5f22054050918f429d6992469a
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14248247
DOI:10.3390/ph16081108