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Growth Performance of a Newly Isolated and Culturable Thraustochytrid Strain from Sea Squirt Colonies

التفاصيل البيبلوغرافية
العنوان: Growth Performance of a Newly Isolated and Culturable Thraustochytrid Strain from Sea Squirt Colonies
المؤلفون: Andy Qarri, Yuval Rinkevich, Jacob Douek, Aydan Sardogan, Baruch Rinkevich
المصدر: Fishes, Vol 9, Iss 1, p 22 (2024)
بيانات النشر: MDPI AG, 2024.
سنة النشر: 2024
المجموعة: LCC:Biology (General)
LCC:Genetics
مصطلحات موضوعية: thraustochytrid strains, marine invertebrates, cultivation, inoculation, isolation, cell culture medium, Biology (General), QH301-705.5, Genetics, QH426-470
الوصف: The world’s oceans and seas host >100 known strains of thraustochytrids, a common group of marine eukaryotic unicellular protists, residing in diverse marine habitats, with many others to be isolated and cultivated. The thraustochytrids have become of considerable industrial interest due to health benefits gained from their high percentages of valuable bioactive compounds, revealing the needs for the isolation of new potential strains. Employing a recently developed isolation methodology (use of cell culture medium), we assess initial culture conditions and growth paces of newly isolated thraustochytrid cells (thraustochytrid sp. BSH), originated from the colonial tunicate Botryllus schlosseri, residing on Helgoland Island, Germany. Cells were cultivated under static versus agitated conditions, along with two inoculation sizes (0.5 × 106 and 1 × 106 cells/dish) and in three vessel types (35 mm Petri dishes and T25 and T75 flasks; containing 3, 5 and 15 mL medium, respectively). Cultures were observed under regular microscopy, confocal microscopy and H&E staining. While cells in all conditions grew fast, results revealed the superiority of agitated cultivation in T75 flasks inoculated with 0.5 × 106 cells/dish (6.41 ± 1.91-fold increase/week). Further, 18S rDNA revealed high similarities (99.5–99.8) of strain BSH to two thraustochytrid strains from Monterey, California (USA), B. schlosseri colonies, elucidating a new understanding of these animals-protists associations.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2410-3888
Relation: https://www.mdpi.com/2410-3888/9/1/22; https://doaj.org/toc/2410-3888
DOI: 10.3390/fishes9010022
URL الوصول: https://doaj.org/article/6122f544f226437087234177844507a9
رقم الأكسشن: edsdoj.6122f544f226437087234177844507a9
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:24103888
DOI:10.3390/fishes9010022