دورية أكاديمية
Induction of annexin-1 at transcriptional and post-transcriptional level in rat brain by methylprednisolone and the 21-aminosteroid U74389F
| العنوان: | Induction of annexin-1 at transcriptional and post-transcriptional level in rat brain by methylprednisolone and the 21-aminosteroid U74389F |
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| المؤلفون: | P. H. Voermans, K. G. Go, G. J. Ter Horst, M. H. J. Ruiters, E. Solito, L. Parente |
| المصدر: | Mediators of Inflammation, Vol 5, Iss 5, Pp 370-378 (1996) |
| بيانات النشر: | Wiley, 1996. |
| سنة النشر: | 1996 |
| المجموعة: | LCC:Pathology |
| مصطلحات موضوعية: | Pathology, RB1-214 |
| الوصف: | Brain tissue of rats pretreated with methylprednisolone or with the 21-aminosteroid U74389F, and that of untreated control rats, was assessed for the expression of annexin-1 (Anx-1) and the transcription of its mRNA. For this purpose Anx-1 cDNA was amplified and simultaneously a T7-RNA-polymerase promoter was incorporated into the cDNA using a polymerase chain reaction (PCR). Then digoxigenin-11-UTP was incorporated into the transcribed cRNA with T7-RNA-polymerase. With this probe in situ hybridization was carried out on sections of the brain. The probe was visualized by an immunoassay using an antidigoxigenin antibody conjugate. Anx-1 protein was assessed by means of immunohistochemistry using a polyclonal antibody. The various brain areas of the control animals showed an appreciable amount of Anx-1 at mRNA or protein level; on the other hand, the animals which had been pretreated with either steroid, showed a more intense Anx-1 mRNA signal than the controls in many areas. In the pretreated animals Anx-1 immunostaining was unchanged in cortex, basal ganglia, amygdala and septum, but more intense in hippocampus, hypothalamus and thalamus. In ependyma, choroid plexus, meninges, and vascular walls there was no Anx-1 mRNA transcription detectable. An opposite profile was shown by the Anx-1 immunoreactivity, the protein was present in control animals as well as the steroid-pretreated animals, suggesting that here the protein was either from systemic origin, or has diffused from adjacent structures. The results indicated that Anx-1 mRNA transcription is upregulated by either steroid, and that in the untreated animals there is a resting level of Anx-1 mRNA transcription, presumably reflecting physiological influences on Anx-1 expression. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0962-9351 1466-1861 09629351 |
| Relation: | https://doaj.org/toc/0962-9351; https://doaj.org/toc/1466-1861 |
| DOI: | 10.1155/S0962935196000531 |
| URL الوصول: | https://doaj.org/article/dca6146a3d2646dbb7000a69730e3053 |
| رقم الأكسشن: | edsdoj.6146a3d2646dbb7000a69730e3053 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 09629351 14661861 |
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| DOI: | 10.1155/S0962935196000531 |