دورية أكاديمية
The gephyrin scaffold modulates cortical layer 2/3 pyramidal neuron responsiveness to single whisker stimulation
| العنوان: | The gephyrin scaffold modulates cortical layer 2/3 pyramidal neuron responsiveness to single whisker stimulation |
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| المؤلفون: | Yuan-Chen Tsai, Mohammad Hleihil, Kanako Otomo, Andrin Abegg, Anna Cavaccini, Patrizia Panzanelli, Teresa Cramer, Kim David Ferrari, Matthew J. P. Barrett, Giovanna Bosshard, Theofanis Karayannis, Bruno Weber, Shiva K. Tyagarajan, Jillian L. Stobart |
| المصدر: | Scientific Reports, Vol 14, Iss 1, Pp 1-15 (2024) |
| بيانات النشر: | Nature Portfolio, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | GABAA receptors, Gephyrin, Barrel cortex, Electrophysiology, Two-photon calcium imaging, Medicine, Science |
| الوصف: | Abstract Gephyrin is the main scaffolding protein at inhibitory postsynaptic sites, and its clusters are the signaling hubs where several molecular pathways converge. Post-translational modifications (PTMs) of gephyrin alter GABAA receptor clustering at the synapse, but it is unclear how this affects neuronal activity at the circuit level. We assessed the contribution of gephyrin PTMs to microcircuit activity in the mouse barrel cortex by slice electrophysiology and in vivo two-photon calcium imaging of layer 2/3 (L2/3) pyramidal cells during single-whisker stimulation. Our results suggest that, depending on the type of gephyrin PTM, the neuronal activities of L2/3 pyramidal neurons can be differentially modulated, leading to changes in the size of the neuronal population responding to the single-whisker stimulation. Furthermore, we show that gephyrin PTMs have their preference for selecting synaptic GABAA receptor subunits. Our results identify an important role of gephyrin and GABAergic postsynaptic sites for cortical microcircuit function during sensory stimulation. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2045-2322 |
| Relation: | https://doaj.org/toc/2045-2322 |
| DOI: | 10.1038/s41598-024-54720-7 |
| URL الوصول: | https://doaj.org/article/61f06bd1f1de40d1b7c391b161388674 |
| رقم الأكسشن: | edsdoj.61f06bd1f1de40d1b7c391b161388674 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-024-54720-7 |