دورية أكاديمية
Protective effect of melatonin on blood⁃brain barrier dysfunction in rats with chronic cerebral hypoperfusion
| العنوان: | Protective effect of melatonin on blood⁃brain barrier dysfunction in rats with chronic cerebral hypoperfusion |
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| المؤلفون: | Shao⁃peng WANG, Ru WANG, Dan⁃dan LI, Hong⁃yi ZHAO, Li⁃yi CHI, Yong⁃hua HUANG |
| المصدر: | Chinese Journal of Contemporary Neurology and Neurosurgery, Vol 19, Iss 8, Pp 552-558 (2019) |
| بيانات النشر: | Tianjin Huanhu Hospital, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Neurology. Diseases of the nervous system |
| مصطلحات موضوعية: | Cerebral small vessel diseases, Melatonin, Blood⁃brain barrier, Disease models, animal, Neurology. Diseases of the nervous system, RC346-429 |
| الوصف: | Objective To investigate the mechanism of the effect of melatonin (MT) on permeability of blood-brainbarrier (BBB) in chronic cerebral hypoperfusion (CCH) rats. Methods Therat model of CCH was established by bilateral common carotid artery occlusion (BCCAO). A total of 72 male Sprague-Dawley rats were randomly divided into 4 groups:sham group(N=18),BCCAOgroup(N=18), the melatonin [5 mg/(kg·d)] treatment model group (MT1 group, N=18), and the melatonin [10 mg/(kg·d)] treatment model group(MT2 group,N=18). Evans blue staining and fluorescein-labeled glucoside(FITC-Dextran)staining marked by fluorescein isothiocyanate(FITC)were used to assess the permeability o fBBB in rats. Expression of matrix metalloproteinase-2(MMP-2) and MMP-9 mRNA levels in basal ganglia of the brain were measured by quantitative real-time polymerase chain reaction (qRT-PCR). Expression of Occludin and Claudin-5 protein in rat basal ganglia were measured by Western blotting method. Results Compared with the sham group, the fluorescence density in basal ganglia was increased (P = 0.000), expression of MMP-2 and MMP-9 were increased(all P=0.000),and expression of Occludin and Claudin-5 were decreased (all P =0.000) of BCCAO group. Compared with the BCCAO group, the fluorescence density in basal ganglia was decreased (P=0.021),the expression of MMP-2 was decreased(P=0.000)and the expression of Claudin-5 was increased (P=0.000) in MT1 group, and the differences with sham group were statistically significant (P=0.000, 0.006, 0.000). Compared with the BCCAO group, in MT2 group,the fluorescence degree in basal ganglia was further decreased (P=0.000), the expression of MMP-2 and MMP-9 were decreased (all P=0.000),and were similar to the sham group (all P>0.05),while expression of Occludin and Claudin-5 were increased (all P=0.000) but lower than those of sham group (P=0.003, 0.000). Compared with the two melatonin treatment groups, the efficacy of the 10mg/(kg·d) group (MT2 group)was better thanthat of the 5mg/(kg·d ) group (MT1 group, all P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Chinese |
| تدمد: | 1672-6731 |
| Relation: | http://www.cjcnn.org/index.php/cjcnn/article/view/1993; https://doaj.org/toc/1672-6731 |
| DOI: | 10.3969/j.issn.1672⁃6731.2019.08.004 |
| URL الوصول: | https://doaj.org/article/cdd6349ab8624823ad651e05a9bbbf24 |
| رقم الأكسشن: | edsdoj.6349ab8624823ad651e05a9bbbf24 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16726731 |
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| DOI: | 10.3969/j.issn.1672⁃6731.2019.08.004 |