دورية أكاديمية
Enhancing structural plasticity of PC12 neurons during differentiation and neurite regeneration with a catalytically inactive mutant version of the zRICH protein
| العنوان: | Enhancing structural plasticity of PC12 neurons during differentiation and neurite regeneration with a catalytically inactive mutant version of the zRICH protein |
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| المؤلفون: | Ashoka C. Bandla, Aditya S. Sheth, Sara M. Zarate, Suraj Uskamalla, Elizabeth C. Hager, Victor A. Villarreal, Maribel González-García, Rafael P. Ballestero |
| المصدر: | BMC Neuroscience, Vol 24, Iss 1, Pp 1-15 (2023) |
| بيانات النشر: | BMC, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Neurosciences. Biological psychiatry. Neuropsychiatry LCC:Neurophysiology and neuropsychology |
| مصطلحات موضوعية: | Axon Regeneration, Neuron differentiation, Neuritogenesis, 2’,3’-Cyclic Nucleotide 3’-Phosphodiesterase, Teleost, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571, Neurophysiology and neuropsychology, QP351-495 |
| الوصف: | Abstract Background Studies of the molecular mechanisms of nerve regeneration have led to the discovery of several proteins that are induced during successful nerve regeneration. RICH proteins were identified as proteins induced during the regeneration of the optic nerve of teleost fish. These proteins are 2’,3’-cyclic nucleotide, 3’-phosphodiesterases that can bind to cellular membranes through a carboxy-terminal membrane localization domain. They interact with the tubulin cytoskeleton and are able to enhance neuronal structural plasticity by promoting the formation of neurite branches. Results PC12 stable transfectant cells expressing a fusion protein combining a red fluorescent protein with a catalytically inactive mutant version of zebrafish RICH protein were generated. These cells were used as a model to analyze effects of the protein on neuritogenesis. Differentiation experiments showed a 2.9 fold increase in formation of secondary neurites and a 2.4 fold increase in branching points. A 2.2 fold increase in formation of secondary neurites was observed in neurite regeneration assays. Conclusions The use of a fluorescent fusion protein facilitated detection of expression levels. Two computer-assisted morphometric analysis methods indicated that the catalytically inactive RICH protein induced the formation of branching points and secondary neurites both during differentiation and neurite regeneration. A procedure based on analysis of random field images provided comparable results to classic neurite tracing methods. Graphical Abstract |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1471-2202 |
| Relation: | https://doaj.org/toc/1471-2202 |
| DOI: | 10.1186/s12868-023-00808-1 |
| URL الوصول: | https://doaj.org/article/64aa74b037034903a369d5ba1daf5d24 |
| رقم الأكسشن: | edsdoj.64aa74b037034903a369d5ba1daf5d24 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 14712202 |
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| DOI: | 10.1186/s12868-023-00808-1 |