دورية أكاديمية
Systematic Investigation and Expression Profiles of the Nitrate Transporter 1/Peptide Transporter Family (NPF) in Tea Plant (Camellia sinensis)
| العنوان: | Systematic Investigation and Expression Profiles of the Nitrate Transporter 1/Peptide Transporter Family (NPF) in Tea Plant (Camellia sinensis) |
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| المؤلفون: | Yongxin Wang, Kang Wei, Li Ruan, Peixian Bai, Liyun Wu, Liyuan Wang, Hao Cheng |
| المصدر: | International Journal of Molecular Sciences, Vol 23, Iss 12, p 6663 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | Camellia sinensis, gene expression, nitrate, NPF, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | NRT1/PTR FAMILY (NPF) genes are characterized as nitrate and peptide transporters that played important roles in various substrates transport in plants. However, little is known about the NPF gene in tea plants. Here, a total of 109 CsNPF members were identified from the tea plant genome, and divided into 8 groups according to their sequence characteristics and phylogenetic relationship. Gene structure and conserved motif analysis supported the evolutionary conservation of CsNPFs. Many hormone and stress response cis-acting elements and transcription factor binding sites were found in CsNPF promoters. Syntenic analysis suggested that multiple duplication types contributed to the expansion of NPF gene family in tea plants. Selection pressure analysis showed that CsNPF genes experienced strong purifying selective during the evolution process. The distribution of NPF family genes revealed that 8 NPF subfamilies were formed before the divergence of eudicots and monocots. Transcriptome analysis showed that CsNPFs were expressed differently in different tissues of the tea plant. The expression of 20 CsNPF genes at different nitrate concentrations was analyzed, and most of those genes responded to nitrate resupply. Subcellular localization showed that both CsNPF2.3 and CsNPF6.1 were localized in the plasma membrane, which was consistent with the characteristics of transmembrane proteins involved in NO3- transport. This study provides a theoretical basis for further investigating the evolution and function of NPF genes. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 23126663 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/23/12/6663; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms23126663 |
| URL الوصول: | https://doaj.org/article/a64ec24c8a01467b9b60648cb4dbb9bd |
| رقم الأكسشن: | edsdoj.64ec24c8a01467b9b60648cb4dbb9bd |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23126663 14220067 16616596 |
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| DOI: | 10.3390/ijms23126663 |