دورية أكاديمية
Cigarette smoke extract affects methylation status and attenuates Sca-1 expression of mouse endothelial progenitor cell in vitro
| العنوان: | Cigarette smoke extract affects methylation status and attenuates Sca-1 expression of mouse endothelial progenitor cell in vitro |
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| المؤلفون: | Zhi-Hui He, Yan Chen, Ping Chen, Li-Hua Xie, Gui-Bin Liang, Hong-Liang Zhang, Huai-Huai Peng |
| المصدر: | Tobacco Induced Diseases, Vol 19, Iss January, Pp 1-10 (2021) |
| بيانات النشر: | European Publishing, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Diseases of the respiratory system LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| مصطلحات موضوعية: | methylation, stem cell antigen-1, dysfunction, endothelial progenitor cell, cigarette smoke, Diseases of the respiratory system, RC705-779, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| الوصف: | Introduction Endothelial dysfunction appears in many smoking-related diseases, it is also an important pathophysiological feature. Endothelial progenitor cells (EPCs) are precursors of endothelial cells and have a crucial effect on the repair and maintenance of endothelial integrity. Sca-1 is not only common in bone marrow-derived hematopoietic stem cells (HSCs), but it is also expressed in nonhematopoietic organs by tissue-resident stem and progenitor cells. The aim of this study is to investigate the impact of cigarette smoke extract (CSE) on the function of bone marrow-derived EPCs and the expression level of Sca-1 in EPCs, and also whether the methylation of Sca-1 is involved in EPC dysfunction. Methods We measured EPC capacities including adhesion, secretion and proliferation, the concentration of endothelial nitric oxide synthase (eNOS) and apoptosis-inducing factor (AIF) in cell culture supernatant, and also Sca-1 expression and promoter methylation in EPCs induced by CSE. Decitabine (Dec) was applied to test whether it could alter the impact caused by CSE. Results The adhesion, proliferation and secretion ability of EPCs can be induced to be decreased by CSE in vitro, accompanied by decreased concentrations of AIF and eNOS in cell culture supernatant and decreased Sca-1 expression in EPCs. In addition, Dec could partly attenuate the impact described above. There were no significant differences in the quantitative analysis of Sca-1 promoter methylation among different groups. Conclusions The decreased Sca-1 expression was related to EPC dysfunction induced by CSE. EPC dysfunction resulting from CSE may be related to methylation mechanism, but not the methylation of Sca-1 promoter. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1617-9625 |
| Relation: | http://www.tobaccoinduceddiseases.org/Cigarette-smoke-extract-affects-methylation-status-and-nattenuates-Sca-1-expression,131625,0,2.html; https://doaj.org/toc/1617-9625 |
| DOI: | 10.18332/tid/131625 |
| URL الوصول: | https://doaj.org/article/65fb72acce2f4026b52b7f2ecd9d3b79 |
| رقم الأكسشن: | edsdoj.65fb72acce2f4026b52b7f2ecd9d3b79 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16179625 |
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| DOI: | 10.18332/tid/131625 |