دورية أكاديمية
Impaired immune response and barrier function in GSPD-1-deficient C. elegans infected with Klebsiella pneumoniae
| العنوان: | Impaired immune response and barrier function in GSPD-1-deficient C. elegans infected with Klebsiella pneumoniae |
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| المؤلفون: | Wan-Hua Yang, Po-Hsiang Chen, Hung-Hsin Chang, Hong Luen Kwok, Arnold Stern, Po-Chi Soo, Jiun-Han Chen, Hung-Chi Yang |
| المصدر: | Current Research in Microbial Sciences, Vol 4, Iss , Pp 100181- (2023) |
| بيانات النشر: | Elsevier, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Microbiology LCC:Genetics |
| مصطلحات موضوعية: | Klebsiella pneumoniae, Bacterial colonization, Tight junction, GSPD-1 C. elegans, Microbiology, QR1-502, Genetics, QH426-470 |
| الوصف: | gspd-1-RNAi knockdown Caenorhabditis elegans was used as an immune-compromised model to investigate the role of G6PD in host-pathogen interactions. A shorted lifespan, increased bacterial burden and bacterial translocation were observed in gspd-1-knockdown C. elegans infected with Klebsiella pneumoniae (KP). RNAseq revealed that the innate immune pathway, including clc-1 and tsp-1, was affected by gspd-1 knockdown. qPCR confirmed that tight junction (zoo-1, clc-1) and immune-associated genes (tsp-1) were down-regulated in gspd-1-knockdown C. elegans and following infection with KP. The down-regulation of antimicrobial effector lysozymes, including lys-1, lys-2, lys-7, lys-8, ilys-2 and ilys-3, was found in gspd-1-knockdown C. elegans infected with KP. Deletion of clc-1, tsp-1, lys-7, and daf-2 in gspd-1-knockdown C. elegans infected with KP abolished the shorten lifespan seen in the Mock control. GSPD-1 deficiency in C. elegans resulted in bacterial accumulation and lethality, possibly due to a defective immune response. These findings indicate that GSPD-1 has a protective role in microbial defense in C. elegans by preventing bacterial colonization through bacterial clearance. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2666-5174 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2666517423000020; https://doaj.org/toc/2666-5174 |
| DOI: | 10.1016/j.crmicr.2023.100181 |
| URL الوصول: | https://doaj.org/article/665a025fc0774dada1169d5aeb36476b |
| رقم الأكسشن: | edsdoj.665a025fc0774dada1169d5aeb36476b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 26665174 |
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| DOI: | 10.1016/j.crmicr.2023.100181 |