دورية أكاديمية
MT-Feeding-Induced Impermanent Sex Reversal in the Orange-Spotted Grouper during Sex Differentiation
| العنوان: | MT-Feeding-Induced Impermanent Sex Reversal in the Orange-Spotted Grouper during Sex Differentiation |
|---|---|
| المؤلفون: | Qing Wang, Minwei Huang, Cheng Peng, Xiang Wang, Ling Xiao, Dengdong Wang, Jiaxing Chen, Huihong Zhao, Haifa Zhang, Shuisheng Li, Huirong Yang, Yun Liu, Haoran Lin, Yong Zhang |
| المصدر: | International Journal of Molecular Sciences, Vol 19, Iss 9, p 2828 (2018) |
| بيانات النشر: | MDPI AG, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | grouper, precocious sex reversal, hormone, efferent duct, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | In this study, we systematically investigated the process of sex reversal induced by 17-methyltestosterone (MT) feeding and MT-feeding withdrawal at the ovary differentiation stage in orange-spotted groupers, Epinephelus coioides. Gonadal histology showed that MT feeding induced a precocious sex reversal from immature ovaries to testes, bypassing the formation of an ovarian cavity, and MT-feeding withdrawal led to an ovarian fate. In both the MT feeding and MT-feeding withdrawal phases, cytochrome P450 family 11 subfamily B (cyp11b) gene expression and serum 11-KT levels were not significantly changed, suggesting that the MT-treated fish did not generate endogenous steroids, even though active spermatogenesis occurred. Finally, by tracing doublesex-expressing and Mab-3-related transcription factor 1 (dmrt1)-expressing cells and TUNEL (terminal deoxynucleotidyl transferase 2-deoxyuridine, 5-triphosphate nick end labeling) assays, we found that the efferent duct formed first, and then, the germ cells and somatic cells of the testicular tissue were generated around the efferent duct during MT-feeding-induced precocious sex reversal. Collectively, our findings provide insights into the molecular and cellular mechanisms underlying sex reversal induced by exogenous hormones during sex differentiation in the protogynous orange-spotted grouper. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 |
| Relation: | http://www.mdpi.com/1422-0067/19/9/2828; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms19092828 |
| URL الوصول: | https://doaj.org/article/667818b305a74e6894663a34f13d54e3 |
| رقم الأكسشن: | edsdoj.667818b305a74e6894663a34f13d54e3 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 |
|---|---|
| DOI: | 10.3390/ijms19092828 |