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Indian Gooseberry and Barley Sprout Mixture Inhibits Adipogenesis and Lipogenesis Activity in 3T3-L1 Adipocytes

التفاصيل البيبلوغرافية
العنوان: Indian Gooseberry and Barley Sprout Mixture Inhibits Adipogenesis and Lipogenesis Activity in 3T3-L1 Adipocytes
المؤلفون: Soo-Jeung Park, Dakyung Kim, Jong-Lae Kim, Mi-Ryeong Park, Tae Gi Kim, Ok-Kyung Kim, Da-Eun Nam
المصدر: Applied Sciences, Vol 10, Iss 24, p 9078 (2020)
بيانات النشر: MDPI AG, 2020.
سنة النشر: 2020
المجموعة: LCC:Technology
LCC:Engineering (General). Civil engineering (General)
LCC:Biology (General)
LCC:Physics
LCC:Chemistry
مصطلحات موضوعية: Indian gooseberry, barley sprout, 3T3-L1, adipogenesis, lipogenesis, Technology, Engineering (General). Civil engineering (General), TA1-2040, Biology (General), QH301-705.5, Physics, QC1-999, Chemistry, QD1-999
الوصف: This study aimed to confirm the synergistic effect of an Indian gooseberry (IG) and barley sprout (BP) mixture in differentiated adipocytes. To this end, 3T3-L1 adipocytes were treated with IG, BP, and IGBP mixtures during the differentiation period. On the last day of differentiation, we measured intracellular cAMP, triglyceride (TG), and fatty acid (FA) levels, as well as performed Oil Red O staining, glycerol release, and Western blot assays. During adipogenesis, IGBP (200 μg/mL) increased the cAMP levels by more than 2-fold and decreased the protein expressions levels of p-CREB (66.3%), C/EBPα (79.4%), C/EBPβ (85.9%), and PPARγ (74.1%) compared to those in the C group. Furthermore, the expression levels of the adipogenesis-related genes and GLUT4 (more than 3-fold) were regulated. During lipogenesis, the IGBP (200 μg/mL) activated AMPK and ACC levels and reduced the protein expression levels of SREBP1c, FAS, and LPL. This reduced the FA and TG contents in the cells by 47.6% and 76.3%, respectively, compared to those in the differentiated control (C) group, resulting in a more than 5-fold increase in glycerol release. In conclusion, we found that IGBP inhibited TG synthesis during adipogenesis and lipogenesis, and thus, displayed potential as a functional health food for preventing obesity.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2076-3417
Relation: https://www.mdpi.com/2076-3417/10/24/9078; https://doaj.org/toc/2076-3417
DOI: 10.3390/app10249078
URL الوصول: https://doaj.org/article/667ea7d669f54097ab1401c332a10805
رقم الأكسشن: edsdoj.667ea7d669f54097ab1401c332a10805
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20763417
DOI:10.3390/app10249078