دورية أكاديمية
Species-specificity of the BamA component of the bacterial outer membrane protein-assembly machinery.
العنوان: | Species-specificity of the BamA component of the bacterial outer membrane protein-assembly machinery. |
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المؤلفون: | Elena B Volokhina, Jan Grijpstra, Frank Beckers, Erika Lindh, Viviane Robert, Jan Tommassen, Martine P Bos |
المصدر: | PLoS ONE, Vol 8, Iss 12, p e85799 (2013) |
بيانات النشر: | Public Library of Science (PLoS), 2013. |
سنة النشر: | 2013 |
المجموعة: | LCC:Medicine LCC:Science |
مصطلحات موضوعية: | Medicine, Science |
الوصف: | The BamA protein is the key component of the Bam complex, the assembly machinery for outer membrane proteins (OMP) in gram-negative bacteria. We previously demonstrated that BamA recognizes its OMP substrates in a species-specific manner in vitro. In this work, we further studied species specificity in vivo by testing the functioning of BamA homologs of the proteobacteria Neisseria meningitidis, Neisseria gonorrhoeae, Bordetella pertussis, Burkholderia mallei, and Escherichia coli in E. coli and in N. meningitidis. We found that no BamA functioned in another species than the authentic one, except for N. gonorrhoeae BamA, which fully complemented a N. meningitidis bamA mutant. E. coli BamA was not assembled into the N. meningitidis outer membrane. In contrast, the N. meningitidis BamA protein was assembled into the outer membrane of E. coli to a significant extent and also associated with BamD, an essential accessory lipoprotein of the Bam complex.Various chimeras comprising swapped N-terminal periplasmic and C-terminal membrane-embedded domains of N. meningitidis and E. coli BamA proteins were also not functional in either host, although some of them were inserted in the OM suggesting that the two domains of BamA need to be compatible in order to function. Furthermore, conformational analysis of chimeric proteins provided evidence for a 16-stranded β-barrel conformation of the membrane-embedded domain of BamA. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1932-6203 |
Relation: | http://europepmc.org/articles/PMC3869937?pdf=render; https://doaj.org/toc/1932-6203 |
DOI: | 10.1371/journal.pone.0085799 |
URL الوصول: | https://doaj.org/article/66e90b2e9f89457ebf57f7921dca3f54 |
رقم الأكسشن: | edsdoj.66e90b2e9f89457ebf57f7921dca3f54 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 19326203 |
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DOI: | 10.1371/journal.pone.0085799 |