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Functionalized Electrospun Poly(Vinyl Alcohol) Nanofibrous Membranes with Poly(Methyl Vinyl Ether-Alt-Maleic Anhydride) for Protein Adsorption

التفاصيل البيبلوغرافية
العنوان: Functionalized Electrospun Poly(Vinyl Alcohol) Nanofibrous Membranes with Poly(Methyl Vinyl Ether-Alt-Maleic Anhydride) for Protein Adsorption
المؤلفون: Mesbah Najafi, Joronia Chery, Margaret M. Frey
المصدر: Materials, Vol 11, Iss 6, p 1002 (2018)
بيانات النشر: MDPI AG, 2018.
سنة النشر: 2018
المجموعة: LCC:Technology
LCC:Electrical engineering. Electronics. Nuclear engineering
LCC:Engineering (General). Civil engineering (General)
LCC:Microscopy
LCC:Descriptive and experimental mechanics
مصطلحات موضوعية: electrospinning, protein, adsorption, separation, polymer, nanofiber, Technology, Electrical engineering. Electronics. Nuclear engineering, TK1-9971, Engineering (General). Civil engineering (General), TA1-2040, Microscopy, QH201-278.5, Descriptive and experimental mechanics, QC120-168.85
الوصف: In this work, electrospun poly(vinyl alcohol) (PVA) nanofiber membranes were functionalized by incorporating poly(methyl vinyl ether-alt-maleic anhydride) (poly(MVE/MA), PMA) for the selective adsorption of proteins. The capture performance was regulated by an optimizing buffer pH, PMA content, and protein concentration. Lysozyme was used as the model protein and a high adsorption capacity of 476.53 ± 19.48 was obtained at pH 6, owing to the electrostatic attraction between the negatively charged nanofibers and positively charged proteins. The large specific surface area, highly open porous structure, and abundant available carboxyl groups contributed to such high adsorption performance. Moreover, the nanofiber membranes exhibited good reusability and good selectivity for positively charged proteins. The obtained results can provide a promising method for the purification of proteins in small analytic devices.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1996-1944
Relation: http://www.mdpi.com/1996-1944/11/6/1002; https://doaj.org/toc/1996-1944
DOI: 10.3390/ma11061002
URL الوصول: https://doaj.org/article/6714beaf114249ddae9af77230ead979
رقم الأكسشن: edsdoj.6714beaf114249ddae9af77230ead979
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19961944
DOI:10.3390/ma11061002