دورية أكاديمية
Post-thyroidectomy hypothyroidism increases the expression and activity of ectonucleotidases in platelets: Possible involvement of reactive oxygen species
| العنوان: | Post-thyroidectomy hypothyroidism increases the expression and activity of ectonucleotidases in platelets: Possible involvement of reactive oxygen species |
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| المؤلفون: | Jucimara Baldissarelli, Micheli M. Pillat, Roberta Schmatz, Andréia M. Cardoso, Fátima H. Abdalla, Juliana S. de Oliveira, Carla R. N. Polachini, Emerson Casali, Clarissa Pereira Bornemann, Henning Ulrich, Vera M. Morsch, Maria R. C. Schetinger |
| المصدر: | Platelets, Vol 29, Iss 8, Pp 801-810 (2018) |
| بيانات النشر: | Taylor & Francis Group, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Diseases of the blood and blood-forming organs |
| مصطلحات موضوعية: | adenosine deaminase, ectonucleotidases, hypothyroidism, oxidative stress, platelets, Diseases of the blood and blood-forming organs, RC633-647.5 |
| الوصف: | Signaling mediated by purines is a widespread mechanism of cell–cell communication related to vasomotor responses and the control of platelet function in the vascular system. However, little is known about the involvement of this signaling as well as the role of reactive oxygen species (ROS) in the development of hypothyroidism. Therefore, the present study investigates changes in the purinergic system, including enzyme activities and expression in platelets, and oxidative profiles in patients with post-thyroidectomy hypothyroidism. The nucleoside triphosphate diphosphohydrolase 1 (NTPDase/CD39) expression in patients increased by 40%, and the adenosine triphosphate (ATP) or adenosine diphosphate (ADP) hydrolyzing activity increased by 82% and 70%, respectively. The activities of ecto-5´-nucleotidase and adenosine deaminase (ADA) also significantly enhanced (39% and 52%, respectively), which correlates with a 45% decrease in adenosine concentration. Furthermore, these patients demonstrated an increased production of ROS (42%), thiobarbituric acid reactive substances (TBARS) (115%), carbonyl protein (30%) and a decreased glutathione S-transferase (GST) activity (20%). This study demonstrates that hypothyroidism interferes with adenine nucleoside and nucleotide hydrolysis and this is correlated with oxidative stress, which might be responsible for the increase in ADA activity. This increase causes rapid adenosine deamination, which can generate a decrease in their concentration in the systemic circulation, which can be associated with the development of vascular complications. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0953-7104 1369-1635 09537104 |
| Relation: | https://doaj.org/toc/0953-7104; https://doaj.org/toc/1369-1635 |
| DOI: | 10.1080/09537104.2017.1361017 |
| URL الوصول: | https://doaj.org/article/67519b2ab29349d89705f7b995c4e950 |
| رقم الأكسشن: | edsdoj.67519b2ab29349d89705f7b995c4e950 |
| قاعدة البيانات: | Directory of Open Access Journals |
PDF full text from Publisher | تدمد: | 09537104 13691635 |
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| DOI: | 10.1080/09537104.2017.1361017 |