دورية أكاديمية
LRG1 promotes epithelial-mesenchymal transition of retinal pigment epithelium cells by activating NOX4
| العنوان: | LRG1 promotes epithelial-mesenchymal transition of retinal pigment epithelium cells by activating NOX4 |
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| المؤلفون: | Li Zhou, De-Peng Shi, Wen-Juan Chu, Ling-Ling Yang, Hai-Feng Xu |
| المصدر: | International Journal of Ophthalmology, Vol 14, Iss 3, Pp 349-355 (2021) |
| بيانات النشر: | Press of International Journal of Ophthalmology (IJO PRESS), 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Ophthalmology |
| مصطلحات موضوعية: | leucine-rich-alpha-2-glycoprotein 1, epithelial-mesenchymal transition, nadph oxidase 4, retinal pigment epithelium cells, subretinal fibrosis, Ophthalmology, RE1-994 |
| الوصف: | AIM: To investigate the effect of leucine-rich-alpha-2-glycoprotein 1 (LRG1) on epithelial-mesenchymal transition (EMT) in retinal pigment epithelium (RPE) cells, and to explore the role of NADPH oxidase 4 (NOX4). METHODS: RPE cells (ARPE-19 cell line) were treated with transforming growth factor-β1 (TGF-β1) to induce EMT. Changes of the mRNA and protein expression levels of LRG1 were tested in the TGF-β1 treated cells. The recombinant human LRG1 protein (rLRG1) and siRNA of LRG1 were used to establish accumulation of exogenous LRG1 model and the down-regulation of LRG1 model in ARPE-19 cells respectively, and to detect EMT-related markers including fibronectin, α-smooth muscle actin (α-SMA) and zonula occludens-1 (ZO-1). The mRNA and protein expression level of NOX4 were measured according to the above treatments. VAS2870 was used as a NOX4 inhibitor in rLRG1-treated cells. EMT-related markers were detected to verify the effect of NOX4 in the process of EMT. RESULTS: TGF-β1 promoted the expression of LRG1 at both the mRNA and protein levels during the process of EMT which showed the up-regulation of fibronectin and α-SMA, as well as the down-regulation of ZO-1. Furthermore, the rLRG1 promoted EMT of ARPE-19 cells, which manifested high levels of fibronectin and α-SMA and low level of ZO-1, whereas knockdown of LRG1 prevented EMT by decreasing the expressions of fibronectin and α-SMA and increasing the expression of ZO-1 in ARPE-19 cells. Besides, the rLRG1 activated and LRG1 siRNA suppressed NOX4 expression. EMT was inhibited when VAS2870 was used in the rLRG1-treated cells. CONCLUSION: These results for the first time demonstrate that LRG1 promotes EMT of RPE cells by activating NOX4, which may provide a novel direction to explore the mechanisms of subretinal fibrosis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2222-3959 2227-4898 |
| Relation: | http://ies.ijo.cn/en_publish/2021/3/20210303.pdf; https://doaj.org/toc/2222-3959; https://doaj.org/toc/2227-4898 |
| DOI: | 10.18240/ijo.2021.03.03 |
| URL الوصول: | https://doaj.org/article/67d59b537bd54414aeced8bc7e668784 |
| رقم الأكسشن: | edsdoj.67d59b537bd54414aeced8bc7e668784 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22223959 22274898 |
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| DOI: | 10.18240/ijo.2021.03.03 |