دورية أكاديمية
Investigation of nanotopography on SOCE mediated cell migration via live-cell Imaging on opaque implant surface
| العنوان: | Investigation of nanotopography on SOCE mediated cell migration via live-cell Imaging on opaque implant surface |
|---|---|
| المؤلفون: | Yan Zhang, Kai Li, Guangwen Li, Yazheng Wang, Yide He, Wen Song, Yumei Zhang |
| المصدر: | Journal of Nanobiotechnology, Vol 21, Iss 1, Pp 1-12 (2023) |
| بيانات النشر: | BMC, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biotechnology LCC:Medical technology |
| مصطلحات موضوعية: | Nanotopography, SOCE, Cell migration, Live-cell imaging, Opaque surface, Stim1, Biotechnology, TP248.13-248.65, Medical technology, R855-855.5 |
| الوصف: | Abstract The exploration of cell response to nanotopography has attracted considerable attentions for years. This article focuses on the influence of nanotopography on the intracellular Ca2+ dynamics, the most ubiquitous but ignored second messenger. The classic titanium nanotubes (NT) were fabricated by anodization to formulate nanoporous surfaces. Firstly, the store operative calcium entry (SOCE) in endoplasmic reticulum (ER) and functional Ca2+ release-activated Ca2+ (CRAC) channels were significantly enhanced on NT surfaces that revealed by live-cell Ca2+ imaging and fluorescence resonance energy transfer (FRET) identification of orai1-stim1 connection. To investigate the potential implication of Ca2+ elevation, the dynamic cell migration trajectory was monitored by a self-made holder, which could not only be suitable for the opaque implant surface but also guarantee the focus fields identical during samples shifting. The cell migration on NT surface was more vigorous and rapid, which was correlated with higher focal adhesion proteins expression, Ca2+-dependent calpain activity and stim1 level. In conclusion, this study has confirmed the novel ER Ca2+ hemostasis pathway on nanosurfaces and its crucial role in cell migration regulation, which may help for more biofavorable implant surface design. Graphical Abstract |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1477-3155 55547559 |
| Relation: | https://doaj.org/toc/1477-3155 |
| DOI: | 10.1186/s12951-023-02249-8 |
| URL الوصول: | https://doaj.org/article/680ac89cf555475592261ffbaa0c6159 |
| رقم الأكسشن: | edsdoj.680ac89cf555475592261ffbaa0c6159 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 14773155 55547559 |
|---|---|
| DOI: | 10.1186/s12951-023-02249-8 |