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Ultrasensitive detection of proteins and sugars at single-cell level

التفاصيل البيبلوغرافية
العنوان: Ultrasensitive detection of proteins and sugars at single-cell level
المؤلفون: Satoshi Watabe, Mika Morikawa, Mugiho Kaneda, Kazunari Nakaishi, Akira Nakatsuma, Masaki Ninomiya, Teruki Yoshimura, Toshiaki Miura, Etsuro Ito
المصدر: Communicative & Integrative Biology, Vol 9, Iss 1 (2016)
بيانات النشر: Taylor & Francis Group, 2016.
سنة النشر: 2016
المجموعة: LCC:Biology (General)
مصطلحات موضوعية: adiponectin, fluorescence correlation spectroscopy, HIV-1 p24, insulin, thio-NAD cycling, Biology (General), QH301-705.5
الوصف: Each cell produces its own responses even if it appears identical to other cells. To analyze these individual cell characteristics, we need to measure trace amounts of molecules in a single cell. Nucleic acids in a single cell can be easily amplified by polymerase chain reaction, but single-cell measurement of proteins and sugars will require de novo techniques. In the present study, we outline the techniques we have developed toward this end. For proteins, our ultrasensitive enzyme-linked immunosorbent assay (ELISA) coupled with thionicotinamide-adenine dinucleotide cycling can detect proteins at subattomoles per assay. For sugars, fluorescence correlation spectroscopy coupled with glucose oxidase-catalyzed reaction allows us to measure glucose at tens of nM. Our methods thus offer versatile techniques for single-cell-level analyses, and they are hoped to strongly promote single-cell biology as well as to develop noninvasive tests in clinical medicine.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1942-0889
19420889
Relation: https://doaj.org/toc/1942-0889
DOI: 10.1080/19420889.2015.1124201
URL الوصول: https://doaj.org/article/e682f16208de4996961d62f55ac8170f
رقم الأكسشن: edsdoj.682f16208de4996961d62f55ac8170f
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19420889
DOI:10.1080/19420889.2015.1124201