دورية أكاديمية
Analysis of alcohol dehydrogenase inhibitors from Desmodium styracifolium using centrifugal ultrafiltration coupled with HPLC-MS
| العنوان: | Analysis of alcohol dehydrogenase inhibitors from Desmodium styracifolium using centrifugal ultrafiltration coupled with HPLC-MS |
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| المؤلفون: | Liu Liangliang, Chen Miao, Chen Xiaoqing |
| المصدر: | Journal of the Serbian Chemical Society, Vol 80, Iss 8, Pp 1051-1059 (2015) |
| بيانات النشر: | Serbian Chemical Society, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Chemistry |
| مصطلحات موضوعية: | alcohol dehydrogenases, aromadendrin, centrifugal ultrafiltration, Desmodium styracifolium, formononetin, Chemistry, QD1-999 |
| الوصف: | Alcohol dehydrogenase (ADH) inhibitors play an important role in the treatment of human methanol or ethylene glycol poisoning and the suppression of acetaldehyde accumulation in alcoholics. In this study, centrifugal ultrafiltration coupled with high performance liquid chromatography-mass spectrometry (HPLC-MS) was utilized to screen and identify ADH inhibitors from ethyl acetate extract of Desmosium styracifolium (Osb.) Merr. The experiment conditions of centrifugal ultrafiltration were optimized. At the optimum conditions (ADH concentration: 37.5 μg mL-1, incubation time: 90 min, pH: 7.0 and temperature: 15°C), formononetin and aromadendrin were successfully screened and identified from ethyl acetate extract of Desmodium styracifolium. The screening result was verified by ADH inhibition assays. The IC50 values of formononetin and aromadendrin were 70.8 and 84.7 μg mL-1, which were accorded with the binding degrees of them. Aromadendrin was first reported to have inhibitory activity on ADH. This method provided an effective way to screen active compounds from natural products. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0352-5139 1820-7421 |
| Relation: | https://doaj.org/toc/0352-5139; https://doaj.org/toc/1820-7421 |
| DOI: | 10.2298/JSC140919023L |
| URL الوصول: | https://doaj.org/article/68472b9b1ef44829b16567e25cd1ec11 |
| رقم الأكسشن: | edsdoj.68472b9b1ef44829b16567e25cd1ec11 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 03525139 18207421 |
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| DOI: | 10.2298/JSC140919023L |