دورية أكاديمية
The Co-Expression Pattern of p63 and HDAC1: A Potential Way to Disclose Stem Cells in Interfollicular Epidermis
| العنوان: | The Co-Expression Pattern of p63 and HDAC1: A Potential Way to Disclose Stem Cells in Interfollicular Epidermis |
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| المؤلفون: | Jung-Won Shin, Hye-Ryung Choi, Kyung-Mi Nam, Hyun-Sun Lee, Sung-Ae Kim, Hyun-Jae Joe, Toyama Kazumi, Kyoung-Chan Park |
| المصدر: | International Journal of Molecular Sciences, Vol 18, Iss 7, p 1360 (2017) |
| بيانات النشر: | MDPI AG, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | epidermal stem cell, HDAC1, interfollicular epidermis, stem cell niche, p63, suberoylanilohydroxamic acid (SAHA), Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Stem cell markers of interfollicular epidermis (IEF) have not been established thus far. The aim of this study is to suggest a new way to disclose IFE-stem cells by combining the expression of histone deacetylases (HDAC) 1 and p63. Immunohistochemical staining of HDAC1 and p63 was performed in six normal human samples. Moreover, a skin equivalent (SE) model was treated with suberoylanilohydroxamic acid (SAHA, an HDAC inhibitor) to elucidate the role of HDAC1. Finally, rapidly adhering (RA) keratinocytes to a type IV collagen, which have been identified to represent epidermal stem cells, were subjected to Western blot analysis with antibodies against HDAC1. In normal samples, there was a minor subpopulation comprised of p63-positive and HDAC1-negative cells in the basal layers. The proportion of this subpopulation was decreased with age. In the SE model, SAHA treatment increased the epidermal thickness and number of p63-positive cells in a dose dependent manner. After SAHA treatment, the expression of differentiation markers was decreased, while that of basement membrane markers was increased. In a Western blot analysis, HDAC1 was not expressed in RA cells. In conclusion, the combination of p63-positive and HDAC1-negative expressions can be a potential new way for distinguishing epidermal stem cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 |
| Relation: | http://www.mdpi.com/1422-0067/18/7/1360; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms18071360 |
| URL الوصول: | https://doaj.org/article/eace6848a85e473c833beb5c7a9982ee |
| رقم الأكسشن: | edsdoj.6848a85e473c833beb5c7a9982ee |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 |
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| DOI: | 10.3390/ijms18071360 |