دورية أكاديمية
Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain
| العنوان: | Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain |
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| المؤلفون: | Makoto Morita, Hidemitsu Sugihara, Kazuhiro Tokunaka, Arihiro Tomura, Kan Saiga, Takamichi Sato, Yasutada Imamura, Toshihiko Hayashi |
| المصدر: | Biochemistry and Biophysics Reports, Vol 9, Iss C, Pp 128-132 (2017) |
| بيانات النشر: | Elsevier, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Biology (General) LCC:Biochemistry |
| مصطلحات موضوعية: | Type IV collagen, Triple-helical conformation, Antibody, Basement membrane, Hydroxyproline, Biology (General), QH301-705.5, Biochemistry, QD415-436 |
| الوصف: | This report describes the preparation and partial characterization of monoclonal antibodies that are reactive specifically with the nascently produced non-triple helical form of the type IV collagen α1 chain, designated as NTH α1(IV). These antibodies were nonreactive with the α1 chain of the type IV collagen in the triple-helical conformation. Three antibodies, #141, #179 and #370, with different epitopes in NTH α1(IV) were found to be reactive with the nascent polypeptide secreted from human normal cells and a human carcinoma cell line. The antibodies with different epitopes may provide a key method for elucidating the physiological function and tissue distribution of NTH α1(IV), which is distinct from the chain derived from triple-helical type IV collagen. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-5808 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405580816303211; https://doaj.org/toc/2405-5808 |
| DOI: | 10.1016/j.bbrep.2016.11.013 |
| URL الوصول: | https://doaj.org/article/6851fb8afb2648ea8cf8a1d751ba9e94 |
| رقم الأكسشن: | edsdoj.6851fb8afb2648ea8cf8a1d751ba9e94 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24055808 |
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| DOI: | 10.1016/j.bbrep.2016.11.013 |