دورية أكاديمية
Phylogenetic Profile of Nonulcerans and Nontuberculous Environmental Mycobacteria Isolated in Côte d’Ivoire
| العنوان: | Phylogenetic Profile of Nonulcerans and Nontuberculous Environmental Mycobacteria Isolated in Côte d’Ivoire |
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| المؤلفون: | Coulibaly Kalpy Julien, Vakou N’dri Sabine, Kouakou Luc Venance, Ouattara Yakoura Karidja, Yao Kouamé Eric, Gnali Gbohounou Fabrice, Dosso Mireille, Djaman Allico Joseph |
| المصدر: | International Journal of Mycobacteriology, Vol 13, Iss 2, Pp 158-164 (2024) |
| بيانات النشر: | Wolters Kluwer Medknow Publications, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Microbiology |
| مصطلحات موضوعية: | abidjan, mycolicibacterium, phylogenetic profile, mycobacteria, mycobacterium phocaicum, wastewater, Microbiology, QR1-502 |
| الوصف: | Background: Environmental mycobacteria are involved in several infections ranging from lung to skin infections. In Côte d’Ivoire, apart from Mycobacterium ulcerans and Mycobacterium tuberculosis, little information exists on other species. The culture of these species, a real challenge, especially in developing countries like Cote d’Ivoire, limits their identification. However, there are reports in literature of infections caused by these mycobacteria, and few species have never been described in human or animal infections. These are difficult cases to treat because of their resistance to most antituberculosis antibiotics. The aim of our work was to study the diversity of potentially pathogenic mycobacterial species in wastewater drainage channels in different townships and in two hospital effluents in the city of Abidjan. Methods: Wastewater samples were cultured, followed by conventional polymerase chain reaction (PCR) targeting mycobacterial 16S ribonucleic acid (16S RNA) using PA/MSHA primers. 16 S RNA identified were sequenced by Sanger techniques. Sequences obtained were analyzed, and a phylogenic tree was built. Results: Fast-growing mycobacteria, including Mycobacterium fortuitum, Mycobacterium phocaicum, Mycobacterium sp., and others presence, were confirmed both by culture and molecular techniques. M. fortuitum strain was the same in effluents of the Treichville University Hospital and in the wastewater of the township of Koumassi. New species never isolated in Côte d’Ivoire, such as M. phocaicum, have been identified in wastewater of the township of Yopougon. Conclusion: This study showed that the sewer network in the city of Abidjan is colonized by both potentially pathogenic mycobacteria and saprophytic environmental mycobacteria. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2212-5531 2212-554X |
| Relation: | https://journals.lww.com/10.4103/ijmy.ijmy_96_24; https://doaj.org/toc/2212-5531; https://doaj.org/toc/2212-554X |
| DOI: | 10.4103/ijmy.ijmy_96_24 |
| URL الوصول: | https://doaj.org/article/68865144ebed4975b50c2d99ad8dd451 |
| رقم الأكسشن: | edsdoj.68865144ebed4975b50c2d99ad8dd451 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22125531 2212554X |
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| DOI: | 10.4103/ijmy.ijmy_96_24 |