دورية أكاديمية
Authentication of Aspergillus parasiticus strains in the genome database of the National Center for Biotechnology Information
| العنوان: | Authentication of Aspergillus parasiticus strains in the genome database of the National Center for Biotechnology Information |
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| المؤلفون: | Perng-Kuang Chang |
| المصدر: | BMC Research Notes, Vol 14, Iss 1, Pp 1-6 (2021) |
| بيانات النشر: | BMC, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine LCC:Biology (General) LCC:Science (General) |
| مصطلحات موضوعية: | Aspergillus parasiticus, Aspergillus flavus, Species identification, Genome, Single nucleotide polymorphism, SU-1, Medicine, Biology (General), QH301-705.5, Science (General), Q1-390 |
| الوصف: | Abstract Objective The use of genome sequences from strains authenticated to correct species level is a prerequisite for confidently exploring the evolutionary relationship among related species. Aspergillus strains erroneously curated as Aspergillus oryzae and Aspergillus fumigatus have been noticed in the National Center for Biotechnology Information (NCBI) genome database. Aspergillus parasiticus is one of several aspergilli that produce aflatoxin, the most potent carcinogenic mycotoxin known up to now. To ensure that valid conclusions are drawn by researchers from their genomics-related studies, molecular analyses were carried out to authenticate identities of A. parasiticus strains in the NCBI genome database. Results Two of the nine supposedly A. parasiticus strains, E1365 and NRRL2999, were found to be misidentified. They turned out to be Aspergillus flavus based on genome-wide single nucleotide polymorphisms (SNPs) and genetic features associated with production of aflatoxin and cyclopiazonic acid. NRRL2999 lacked the additional partial aflatoxin gene cluster known to be present in its equivalent strain, designated as SU-1, and shared a very low total SNPs count specifically with A. flavus NRRL3357 but not with other A. flavus isolates. Therefore, the mislabeled NRRL2999 strain actually is a clonal strain of A. flavus NRRL3357, whose genome was first sequenced in 2005. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1756-0500 |
| Relation: | https://doaj.org/toc/1756-0500 |
| DOI: | 10.1186/s13104-021-05527-6 |
| URL الوصول: | https://doaj.org/article/68e164a17ba7435c8ef747027672a869 |
| رقم الأكسشن: | edsdoj.68e164a17ba7435c8ef747027672a869 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 17560500 |
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| DOI: | 10.1186/s13104-021-05527-6 |