دورية أكاديمية
High-Throughput Screening in Protein Engineering: Recent Advances and Future Perspectives
| العنوان: | High-Throughput Screening in Protein Engineering: Recent Advances and Future Perspectives |
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| المؤلفون: | Magdalena Wójcik, Aline Telzerow, Wim J. Quax, Ykelien L. Boersma |
| المصدر: | International Journal of Molecular Sciences, Vol 16, Iss 10, Pp 24918-24945 (2015) |
| بيانات النشر: | MDPI AG, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | high-throughput screening, FACS-based screening platforms, microfluidics-based screening platforms, protein engineering, in vitro compartmentalization, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Over the last three decades, protein engineering has established itself as an important tool for the development of enzymes and (therapeutic) proteins with improved characteristics. New mutagenesis techniques and computational design tools have greatly aided in the advancement of protein engineering. Yet, one of the pivotal components to further advance protein engineering strategies is the high-throughput screening of variants. Compartmentalization is one of the key features allowing miniaturization and acceleration of screening. This review focuses on novel screening technologies applied in protein engineering, highlighting flow cytometry- and microfluidics-based platforms. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 |
| Relation: | http://www.mdpi.com/1422-0067/16/10/24918; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms161024918 |
| URL الوصول: | https://doaj.org/article/6924ac6633b04849b2cc1f68f26d234c |
| رقم الأكسشن: | edsdoj.6924ac6633b04849b2cc1f68f26d234c |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 |
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| DOI: | 10.3390/ijms161024918 |