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Elevated gaseous luminal nitric oxide and circulating IL-8 as features of Helicobacter pylori-induced gastric inflammation

التفاصيل البيبلوغرافية
العنوان: Elevated gaseous luminal nitric oxide and circulating IL-8 as features of Helicobacter pylori-induced gastric inflammation
المؤلفون: Hiwa K. Saaed, Lisa Chiggiato, Dominic-Luc Webb, Ann-Sofie Rehnberg, Carlos A. Rubio, Ragnar Befrits, Per M. Hellström
المصدر: Upsala Journal of Medical Sciences, Vol 126, Pp 1-9 (2021)
بيانات النشر: Upsala Medical Society, 2021.
سنة النشر: 2021
المجموعة: LCC:Medicine
مصطلحات موضوعية: nitric oxide, helicobacter pylori, inflammation, cytokines, biomarkers, Medicine
الوصف: Background: Gastric nitric oxide (NO) production in response to Helicobacter pylori via inducible nitric oxide synthase (iNOS) is suggested as a biomarker of inflammation and cytotoxicity. The aim of this study was to investigate relationships between gastric [NO], immunological biomarkers and histopathology. Materials and methods: Esophagogastroduodenoscopy was done in 96 dyspepsia patients. Luminal [NO] was measured by chemiluminescence. Biopsies were taken from gastric antrum and corpus for culture and histopathology. H. pylori IgG was detected by immunoblot assay. Biobanked plasma from 76 dyspepsia patients (11 H. pylori positives) was analyzed for 39 cytokines by multiplexed ELISA. Results: H. pylori-positive patients had higher [NO] (336 ± 26 ppb, mean ± 95% CI, n = 77) than H. pylori-negative patients (128 ± 47 ppb, n = 19) (P < 0.0001). Histopathological changes were found in 99% of H. pylori-positive and 37% of H. pylori-negative patients. Histopathological concordance was 78–100% between corpus and antrum. Correlations were found between gastric [NO] and severity of acute, but not chronic, inflammation. Plasma IL-8 (increased in H. pylori positives) had greatest difference between positive and negative groups, with eotaxin, MIP-1β, MCP-4, VEGF-A, and VEGF-C also higher (P < 0.004 to P < 0.032). Diagnostic odds ratios using 75% cut-off concentration were 7.53 for IL-8, 1.15 for CRP, and 2.88 for gastric NO. Conclusions: Of the parameters tested, increased gastric [NO] and circulating IL-8 align most consistently and selectively in H. pylori-infected patients. Severity of mucosal inflammatory changes is proportional to luminal [NO], which might be tied to IL-8 production. It is proposed that IL-8 be further investigated as a blood biomarker of treatment outcomes.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 0300-9734
2000-1967
Relation: https://ujms.net/index.php/ujms/article/view/8116/13987; https://doaj.org/toc/0300-9734; https://doaj.org/toc/2000-1967
DOI: 10.48101/ujms.v126.8116
URL الوصول: https://doaj.org/article/69c340234e45456ba22a6df66e9f2363
رقم الأكسشن: edsdoj.69c340234e45456ba22a6df66e9f2363
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:03009734
20001967
DOI:10.48101/ujms.v126.8116