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7α-Hydroxycholesterol induces monocyte/macrophage cell expression of interleukin-8 via C5a receptor.

التفاصيل البيبلوغرافية
العنوان: 7α-Hydroxycholesterol induces monocyte/macrophage cell expression of interleukin-8 via C5a receptor.
المؤلفون: Hyok-Rae Cho, Yonghae Son, Sun-Mi Kim, Bo-Young Kim, Seong-Kug Eo, Young Chul Park, Koanhoi Kim
المصدر: PLoS ONE, Vol 12, Iss 3, p e0173749 (2017)
بيانات النشر: Public Library of Science (PLoS), 2017.
سنة النشر: 2017
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: We investigated effects of 7-oxygenated cholesterol derivatives present in atherosclerotic lesions, 7α-hydroxycholesterol (7αOHChol), 7β-hydroxycholesterol (7βOHChol), and 7-ketocholesterol (7K), on IL-8 expression. Transcript levels of IL-8 and secretion of its corresponding gene product by monocytes/macrophages were enhanced by treatment with 7αOHChol and, to a lesser extent, 7K, but not by 7βOHChol. The 7-oxygenated cholesterol derivatives, however, did not change transcription of the IL-8 gene in vascular smooth muscle cells. 7αOHChol-induced IL-8 gene transcription was inhibited by cycloheximide and Akt1 downregulation, but not by OxPAPC. Expression of C5a receptor was upregulated after stimulation with 7αOHChol, but not with 7K and 7βOHChol, and a specific antagonist of C5a receptor inhibited 7αOHChol-induced IL-8 gene expression in a dose dependent manner. Pharmacological inhibitors of PI3K and MEK almost completely inhibited expression of both IL-8 and cell-surface C5a receptor induced by 7αOHChol. These results indicate that 7-oxygenated cholesterol derivatives have differential effects on monocyte/macrophage expression of IL-8 and C5a receptor and that C5a receptor is involved in 7αOHChol-induced IL-8 expression via PI3K and MEK.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC5360241?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0173749
URL الوصول: https://doaj.org/article/dca69c8c08f848d7911142e81901d11b
رقم الأكسشن: edsdoj.69c8c08f848d7911142e81901d11b
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0173749