دورية أكاديمية
ABT-737, Synergistically Enhances Daunorubicin-Mediated Apoptosis in Acute Myeloid Leukemia Cell Lines
| العنوان: | ABT-737, Synergistically Enhances Daunorubicin-Mediated Apoptosis in Acute Myeloid Leukemia Cell Lines |
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| المؤلفون: | Hassan Dariushnejad, Nosratallah Zarghami, Mohammad Rahmati, Samaneh Ghasemali, Zohreh Sadeghi, Zahra Davoodi, Hossein Jafari Tekab, Masoud Gandomkar Ghalhar |
| المصدر: | Advanced Pharmaceutical Bulletin, Vol 4, Iss 2, Pp 185-189 (2014) |
| بيانات النشر: | Tabriz University of Medical Sciences, 2014. |
| سنة النشر: | 2014 |
| المجموعة: | LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: | Acute myeloid leukemia, Daunorubicin, ABT-737, Combination, Apoptosis, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Purpose: Intensive chemotherapy with daunorubicin (DNR) is associated with serious side effects in acute myeloid leukemia (AML) patients. In this study the effect of small-molecule BH3-mimetic, ABT-737, on the sensitivity of HL60 and U937 AML cell lines was investigated. Methods: The cytotoxic effects of DNR and ABT-737, alone or in combination were assessed using MTT assay and combination index analysis. The effects of treatments on the cell proliferation was determined by trypan blue assay. ELISA cell death assay was used for measurement of apoptosis. Results: IC50 values of DNR and ABT-737 were 2.52 and 0.59 μM for HL-60 cells line and 1.31 and 0.80 μM for U937 cell line at 24 h, respectively. Surprisingly, combination treatment significantly lowered the IC50 values in a synergic manner in both cell lines. Moreover, treatment with a mixture of two agents had more growth inhibition effect relative to the monotherapy. Results of apoptosis assay showed that the cytotoxic effects are related to the enhancement of apoptosis. Conclusion: Our study suggests that ABT-737 synergistically enhances the cytotoxic effect of DNR in AML cell lines and therefore may be useful to overcome chemoresistance of leukemia patients. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2228-5881 2251-7308 |
| Relation: | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-4-185.pdf; https://doaj.org/toc/2228-5881; https://doaj.org/toc/2251-7308 |
| DOI: | 10.5681/apb.2014.027 |
| URL الوصول: | https://doaj.org/article/69cadce1a684483ca4408ad4c9d24293 |
| رقم الأكسشن: | edsdoj.69cadce1a684483ca4408ad4c9d24293 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22285881 22517308 |
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| DOI: | 10.5681/apb.2014.027 |