دورية أكاديمية
Morphological and Genetic Characterization of Eggerthella lenta Bacteriophage PMBT5
| العنوان: | Morphological and Genetic Characterization of Eggerthella lenta Bacteriophage PMBT5 |
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| المؤلفون: | Sabrina Sprotte, Torben S. Rasmussen, Gyu-Sung Cho, Erik Brinks, René Lametsch, Horst Neve, Finn K. Vogensen, Dennis S. Nielsen, Charles M. A. P. Franz |
| المصدر: | Viruses, Vol 14, Iss 8, p 1598 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Microbiology |
| مصطلحات موضوعية: | anaerobe, Eggerthella lenta, virulent phage, Siphoviridae, genome sequence, Microbiology, QR1-502 |
| الوصف: | Eggerthella lenta is a common member of the human gut microbiome. We here describe the isolation and characterization of a putative virulent bacteriophage having E. lenta as host. The double-layer agar method for isolating phages was adapted to anaerobic conditions for isolating bacteriophage PMBT5 from sewage on a strictly anaerobic E. lenta strain of intestinal origin. For this, anaerobically grown E. lenta cells were concentrated by centrifugation and used for a 24 h phage enrichment step. Subsequently, this suspension was added to anaerobically prepared top (soft) agar in Hungate tubes and further used in the double-layer agar method. Based on morphological characteristics observed by transmission electron microscopy, phage PMBT5 could be assigned to the Siphoviridae phage family. It showed an isometric head with a flexible, noncontractile tail and a distinct single 45 nm tail fiber under the baseplate. Genome sequencing and assembly resulted in one contig of 30,930 bp and a mol% GC content of 51.3, consisting of 44 predicted protein-encoding genes. Phage-related proteins could be largely identified based on their amino acid sequence, and a comparison with metagenomes in the human virome database showed that the phage genome exhibits similarity to two distantly related phages. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 14081598 1999-4915 |
| Relation: | https://www.mdpi.com/1999-4915/14/8/1598; https://doaj.org/toc/1999-4915 |
| DOI: | 10.3390/v14081598 |
| URL الوصول: | https://doaj.org/article/69cf68934dda47eba1999e5c366a5227 |
| رقم الأكسشن: | edsdoj.69cf68934dda47eba1999e5c366a5227 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14081598 19994915 |
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| DOI: | 10.3390/v14081598 |