دورية أكاديمية
Identification and analysis of sucrose synthase gene family associated with polysaccharide biosynthesis in Dendrobium catenatum by transcriptomic analysis
| العنوان: | Identification and analysis of sucrose synthase gene family associated with polysaccharide biosynthesis in Dendrobium catenatum by transcriptomic analysis |
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| المؤلفون: | Min Jiang, Shangyun Li, Changling Zhao, Mingfu Zhao, Shaozhong Xu, Guosong Wen |
| المصدر: | PeerJ, Vol 10, p e13222 (2022) |
| بيانات النشر: | PeerJ Inc., 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine LCC:Biology (General) |
| مصطلحات موضوعية: | SUS, Mannose, RNA-Seq, Evolution, Expression analysis, Dendrobium catenatum, Medicine, Biology (General), QH301-705.5 |
| الوصف: | Background Dendrobium catenatum is a valuable traditional medicinal herb with high commercial value. D. catenatum stems contain abundant polysaccharides which are one of the main bioactive components. However, although some genes related to the synthesis of the polysaccharides have been reported, more key genes need to be further elucidated. Results In this study, the contents of polysaccharides and mannose in D. catenatum stems at four developmental stages were compared, and the stems’ transcriptomes were analyzed to explore the synthesis mechanism of the polysaccharides. Many genes involved in starch and sucrose metabolisms were identified by KEGG pathway analysis. Further analysis found that sucrose synthase (SUS; EC 2.4.1.13) gene maybe participated in the polysaccharide synthesis. Hence, we further investigated the genomic characteristics and evolution relationships of the SUS family in plants. The result suggested that the SUS gene of D. catenatum (DcSUS) had undergone the expansion characterized by tandem duplication which might be related to the enrichment of the polysaccharides in D. catenatum stems. Moreover, expression analyses of the DcSUS displayed significant divergent patterns in different tissues and could be divided into two main groups in the stems with four developmental stages. Conclusion In general, our results revealed that DcSUS is likely involved in the metabolic process of the stem polysaccharides, providing crucial clues for exploiting the key genes associated with the polysaccharide synthesis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2167-8359 |
| Relation: | https://peerj.com/articles/13222.pdf; https://peerj.com/articles/13222/; https://doaj.org/toc/2167-8359 |
| DOI: | 10.7717/peerj.13222 |
| URL الوصول: | https://doaj.org/article/69f4245b75dd4950bbb78155fe4fed5d |
| رقم الأكسشن: | edsdoj.69f4245b75dd4950bbb78155fe4fed5d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 21678359 |
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| DOI: | 10.7717/peerj.13222 |