دورية أكاديمية
Horseradish Peroxidase-Encapsulated Hollow Silica Nanospheres for Intracellular Sensing of Reactive Oxygen Species
| العنوان: | Horseradish Peroxidase-Encapsulated Hollow Silica Nanospheres for Intracellular Sensing of Reactive Oxygen Species |
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| المؤلفون: | Hsin-Yi Chen, Si-Han Wu, Chien-Tsu Chen, Yi-Ping Chen, Feng-Peng Chang, Fan-Ching Chien, Chung-Yuan Mou |
| المصدر: | Nanoscale Research Letters, Vol 13, Iss 1, Pp 1-10 (2018) |
| بيانات النشر: | SpringerOpen, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Materials of engineering and construction. Mechanics of materials |
| مصطلحات موضوعية: | Reactive oxygen species, Hollow silica nanospheres, Horseradish peroxidase, Enzyme delivery, Porous materials, Materials of engineering and construction. Mechanics of materials, TA401-492 |
| الوصف: | Abstract Reactive oxygen species (ROS) have crucial roles in cell signaling and homeostasis. Overproduction of ROS can induce oxidative damage to various biomolecules and cellular structures. Therefore, developing an approach capable of monitoring and quantifying ROS in living cells is significant for physiology and clinical diagnoses. Some cell-permeable fluorogenic probes developed are useful for the detection of ROS while in conjunction with horseradish peroxidase (HRP). Their intracellular scenario is however hindered by the membrane-impermeable property of enzymes. Herein, a new approach for intracellular sensing of ROS by using horseradish peroxidase-encapsulated hollow silica nanospheres (designated HRP@HSNs), with satisfactory catalytic activity, cell membrane permeability, and biocompatibility, was prepared via a microemulsion method. These HRP@HSNs, combined with selective probes or targeting ligands, could be foreseen as ROS-detecting tools in specific organelles or cell types. As such, dihydrorhodamine 123-coupled HRP@HSNs were used for the qualitative and semi-quantitative analysis of physiological H2O2 levels in activated RAW 264.7 macrophages. We envision that this HSNs encapsulating active enzymes can be conjugated with selective probes and targeting ligands to detect ROS in specific organelles or cell types of interest. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1931-7573 1556-276X |
| Relation: | http://link.springer.com/article/10.1186/s11671-018-2527-0; https://doaj.org/toc/1931-7573; https://doaj.org/toc/1556-276X |
| DOI: | 10.1186/s11671-018-2527-0 |
| URL الوصول: | https://doaj.org/article/6a685b5c12a64a0ca15f7c34b79175a5 |
| رقم الأكسشن: | edsdoj.6a685b5c12a64a0ca15f7c34b79175a5 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 19317573 1556276X |
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| DOI: | 10.1186/s11671-018-2527-0 |