دورية أكاديمية
Survival of Bacteriophage T4 in Quasi-Pure Ionic Solutions
| العنوان: | Survival of Bacteriophage T4 in Quasi-Pure Ionic Solutions |
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| المؤلفون: | Seiko Hara, Isao Koike |
| المصدر: | Viruses, Vol 15, Iss 8, p 1737 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Microbiology |
| مصطلحات موضوعية: | infectivity, neutral solution, acidic solution, alkaline solution, deprotonation, Microbiology, QR1-502 |
| الوصف: | The preservative qualities of individual ionic compounds impacting the infectivity of T4 virions were elucidated. T4 virions were immersed in quasi-pure ionic solutions prior to the adsorption process, and the plaque forming unit (pfu) values of these were measured following the conventional method. In neutral ionic solutions, the minimum and the optimum concentrations of preservative qualities corresponded with the results obtained from the multi-ionic media/buffers. In acid and alkali solutions, phages show tolerances at a pH range of 5–11 in multi-ionic media/buffers. T4 virions show no tolerance in quasi-pure acid, neutral, and weak alkaline conditions. The preservative quality of T4 virions increased in over 10−1 mM OH− solution, equivalent to a pH value over 10, which corresponds to the pKa of the deprotonation of the DNA bases G and T. Infectivity was lost below 10−1 mM OH− and higher than 10 mM OH−. These results imply that maintaining infectivity of a virion may need the flexibility of the intra-capsid DNA by deprotonation. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 15081737 1999-4915 |
| Relation: | https://www.mdpi.com/1999-4915/15/8/1737; https://doaj.org/toc/1999-4915 |
| DOI: | 10.3390/v15081737 |
| URL الوصول: | https://doaj.org/article/a6a9d25bd1a645ac821257a88aa0f968 |
| رقم الأكسشن: | edsdoj.6a9d25bd1a645ac821257a88aa0f968 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 15081737 19994915 |
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| DOI: | 10.3390/v15081737 |