دورية أكاديمية
miR-584–5p / Ykt6 - mediated autophagy - lysosome - exosome pathway as a critical route affecting the toxic effects of lead on HK-2 cells
| العنوان: | miR-584–5p / Ykt6 - mediated autophagy - lysosome - exosome pathway as a critical route affecting the toxic effects of lead on HK-2 cells |
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| المؤلفون: | Yiren Xiong, Zuqing Hu, Di Ouyang, Meilin Tang, Jiayi He, Shanshan He, Renyi Liu, Zhenjie Gao, Ying Chen, Dalin Hu |
| المصدر: | Ecotoxicology and Environmental Safety, Vol 276, Iss , Pp 116322- (2024) |
| بيانات النشر: | Elsevier, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Environmental pollution LCC:Environmental sciences |
| مصطلحات موضوعية: | Lead, Toxic effects, MiR-584–5p /Ykt6 axis, Autophagy, Lysosomes, Exosomes, Environmental pollution, TD172-193.5, Environmental sciences, GE1-350 |
| الوصف: | Lead is a widespread environmental pollutant with serious adverse effects on human health, but the mechanism underlying its toxicity remains elusive. This study aimed to investigate the role of miR-584–5p / Ykt6 axis in the toxic effect of lead on HK-2 cells and the related mechanism. Our data suggested that lead exposure caused significant cytotoxicity, DNA and chromosome damage to HK-2 cells. Mechanistically, lead exposure down-regulated miR-584–5p and up-regulated Ykt6 expression, consequently, autophagosomal number and autophagic flux increased, lysosomal number and activity decreased, exosomal secretion increased. Interestingly, when miR-584–5p level was enhanced with mimic, autophagosomal number and autophagic flux decreased, lysosomal number and activity increased, ultimately, exosomal secretion was down-regulated, which resulted in significant aggravated toxic effects of lead. Further, directly blocking exosomal secretion with inhibitor GW4869 also resulted in exacerbated toxic effects of lead. Herein, we conclude that miR-584–5p / Ykt6 - mediated autophagy - lysosome - exosome pathway may be a critical route affecting the toxic effects of lead on HK-2 cells. We provide a novel insight into the mechanism underlying the toxicity of lead on human cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0147-6513 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S0147651324003981; https://doaj.org/toc/0147-6513 |
| DOI: | 10.1016/j.ecoenv.2024.116322 |
| URL الوصول: | https://doaj.org/article/6acbff466adf4a01a8a025dd8e9d3dd1 |
| رقم الأكسشن: | edsdoj.6acbff466adf4a01a8a025dd8e9d3dd1 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 01476513 |
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| DOI: | 10.1016/j.ecoenv.2024.116322 |