دورية أكاديمية
Interferon Gamma Enhances Cytoprotective Pathways via Nrf2 and MnSOD Induction in Friedreich’s Ataxia Cells
| العنوان: | Interferon Gamma Enhances Cytoprotective Pathways via Nrf2 and MnSOD Induction in Friedreich’s Ataxia Cells |
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| المؤلفون: | Riccardo Luffarelli, Luca Panarello, Andrea Quatrana, Francesca Tiano, Silvia Fortuni, Alessandra Rufini, Florence Malisan, Roberto Testi, Ivano Condò |
| المصدر: | International Journal of Molecular Sciences, Vol 24, Iss 16, p 12687 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | Friedreich’s ataxia, interferon gamma, cytoprotection, Nrf2, MnSOD, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Friedreich’s ataxia (FRDA) is a rare monogenic disease characterized by multisystem, slowly progressive degeneration. Because of the genetic defect in a non-coding region of FXN gene, FRDA cells exhibit severe deficit of frataxin protein levels. Hence, FRDA pathophysiology is characterized by a plethora of metabolic disruptions related to iron metabolism, mitochondrial homeostasis and oxidative stress. Importantly, an impairment of the antioxidant defences exacerbates the oxidative damage. This appears closely associated with the disablement of key antioxidant proteins, such as the transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) and the mitochondrial superoxide dismutase (MnSOD). The cytokine interferon gamma (IFN-γ) has been shown to increase frataxin expression in FRDA cells and to improve functional deficits in FRDA mice. Currently, IFN-γ represents a potential therapy under clinical evaluation in FRDA patients. Here, we show that IFN-γ induces a rapid expression of Nrf2 and MnSOD in different cell types, including FRDA patient-derived fibroblasts. Our data indicate that IFN-γ signals two separate pathways to enhance Nrf2 and MnSOD levels in FRDA fibroblasts. MnSOD expression increased through an early transcriptional regulation, whereas the levels of Nrf2 are induced by a post-transcriptional mechanism. We demonstrate that the treatment of FRDA fibroblasts with IFN-γ stimulates a non-canonical Nrf2 activation pathway through p21 and potentiates antioxidant responses under exposure to hydrogen peroxide. Moreover, IFN-γ significantly reduced the sensitivity to hydrogen peroxide-induced cell death in FRDA fibroblasts. Collectively, these results indicate the presence of multiple pathways triggered by IFN-γ with therapeutic relevance to FRDA. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 24161268 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/24/16/12687; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms241612687 |
| URL الوصول: | https://doaj.org/article/6af5911f8f3a43c793988cecd58d4265 |
| رقم الأكسشن: | edsdoj.6af5911f8f3a43c793988cecd58d4265 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24161268 14220067 16616596 |
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| DOI: | 10.3390/ijms241612687 |