دورية أكاديمية
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B and T cell response to SARS-CoV-2 vaccination in health care professionals with and without previous COVID-19

التفاصيل البيبلوغرافية
العنوان: B and T cell response to SARS-CoV-2 vaccination in health care professionals with and without previous COVID-19
المؤلفون: Andreas Zollner, Christina Watschinger, Annika Rössler, Maria R. Farcet, Agnes Penner, Vincent Böhm, Sophia J. Kiechl, Gerald Stampfel, Rainer Hintenberger, Herbert Tilg, Robert Koch, Marlies Antlanger, Thomas R. Kreil, Janine Kimpel, Alexander R. Moschen
المصدر: EBioMedicine, Vol 70, Iss , Pp 103539- (2021)
بيانات النشر: Elsevier, 2021.
سنة النشر: 2021
المجموعة: LCC:Medicine
LCC:Medicine (General)
مصطلحات موضوعية: Vaccination, Covid-19, SARS-CoV-2, T cell immunity, Pre-existing immunity, Humoral response, Medicine, Medicine (General), R5-920
الوصف: Summary: Background: In recent months numerous health care professional acquired COVID-19 at the workplace resulting in significant shortages in medical and nursing staff. We investigated how prior COVID-19 affects SARS-CoV-2 vaccination and how such knowledge could facilitate frugal vaccination strategies. Methods: In a cohort of 41 healthcare professionals with (n=14) and without (n=27) previous SARS-CoV-2 infection, we assessed the immune status before, during and after vaccination with BNT162b2. The humoral immune response was assessed by receptor binding domain ELISA and different SARS-CoV-2 neutralisation assays using wildtype and pseudo-typed viruses. T cell immunity against SARS-CoV-2 surface and nucleocapsid peptides were studied using interferon-γ release assays and intracellular flow cytometry. Vaccine-related side effects were captured. Findings: Prior COVID-19 resulted in improved vaccine responses both in the B and T cell compartment. In vaccine recipients with prior COVID-19, the first vaccine dose induced high antibody concentrations comparable to seronegative vaccine recipients after two injections. This translated into more efficient neutralisation of virus particles, even more pronounced than expected from the RBD ELISA results. Furthermore, T cell responses were stronger in convalescents and particularly strong against the SARS-CoV-2 nucleocapsid protein. Interpretation: Herein, we corroborate recent findings suggesting that in convalescents a single vaccine dose is sufficient to boost adequate in vitro neutralisation of SARS-CoV-2 and therefore may be sufficient to induce adequate protection against severe COVID-19. New spike mutated virus variants render the highly conserved nucleocapsid protein – eliciting strong SARS-CoV-2 specific T cell immunity – an interesting additional vaccine target. Funding: Christian Doppler Research Association, Johannes Kepler University Linz
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2352-3964
Relation: http://www.sciencedirect.com/science/article/pii/S2352396421003327; https://doaj.org/toc/2352-3964
DOI: 10.1016/j.ebiom.2021.103539
URL الوصول: https://doaj.org/article/6b224a0898a14ebd9079f3d76d3fc58b
رقم الأكسشن: edsdoj.6b224a0898a14ebd9079f3d76d3fc58b
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:23523964
DOI:10.1016/j.ebiom.2021.103539