دورية أكاديمية
Enhanced Assessment of Cross-Reactive Antigenic Determinants within the Spike Protein
| العنوان: | Enhanced Assessment of Cross-Reactive Antigenic Determinants within the Spike Protein |
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| المؤلفون: | Guilherme C. Lechuga, Jairo R. Temerozo, Paloma Napoleão-Pêgo, João P. R. S. Carvalho, Larissa R. Gomes, Dumith Chequer Bou-Habib, Carlos M. Morel, David W. Provance, Thiago M. L. Souza, Salvatore G. De-Simone |
| المصدر: | International Journal of Molecular Sciences, Vol 25, Iss 15, p 8180 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | antibody-mediated enhancement, B-cell epitope, COVID-19, Dengue virus, SARS-CoV-2 variants, spike protein, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Despite successful vaccination efforts, the emergence of new SARS-CoV-2 variants poses ongoing challenges to control COVID-19. Understanding humoral responses regarding SARS-CoV-2 infections and their impact is crucial for developing future vaccines that are effective worldwide. Here, we identified 41 immunodominant linear B-cell epitopes in its spike glycoprotein with an SPOT synthesis peptide array probed with a pool of serum from hospitalized COVID-19 patients. The bioinformatics showed a restricted set of epitopes unique to SARS-CoV-2 compared to other coronavirus family members. Potential crosstalk was also detected with Dengue virus (DENV), which was confirmed by screening individuals infected with DENV before the COVID-19 pandemic in a commercial ELISA for anti-SARS-CoV-2 antibodies. A high-resolution evaluation of antibody reactivity against peptides representing epitopes in the spike protein identified ten sequences in the NTD, RBD, and S2 domains. Functionally, antibody-dependent enhancement (ADE) in SARS-CoV-2 infections of monocytes was observed in vitro with pre-pandemic Dengue-positive sera. A significant increase in viral load was measured compared to that of the controls, with no detectable neutralization or considerable cell death, suggesting its role in viral entry. Cross-reactivity against peptides from spike proteins was observed for the pre-pandemic sera. This study highlights the importance of identifying specific epitopes generated during the humoral response to a pathogenic infection to understand the potential interplay of previous and future infections on diseases and their impact on vaccinations and immunodiagnostics. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/25/15/8180; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms25158180 |
| URL الوصول: | https://doaj.org/article/d6b8088a6c6a4050b3bba2afb0bcfb93 |
| رقم الأكسشن: | edsdoj.6b8088a6c6a4050b3bba2afb0bcfb93 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms25158180 |