دورية أكاديمية
Suppression of P-cadherin expression as a key regulatory element for embryonic stem cell stemness
| العنوان: | Suppression of P-cadherin expression as a key regulatory element for embryonic stem cell stemness |
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| المؤلفون: | Yuka Takeda, Shuji Matsuguchi, Sae Nozaki, Taisei Mihara, Junya Abe, Yohei Hirai |
| المصدر: | Cell Structure and Function, Vol 48, Iss 1, Pp 49-57 (2022) |
| بيانات النشر: | Japan Society for Cell Biology, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | differentiation, embryoid body, es cells, p-cadherin, syntaxin4, Science, Biology (General), QH301-705.5 |
| الوصف: | In embryonic stem (ES) cell colonies, a small subpopulation that changes cell shape and loses pluripotency often appears in two-dimensional (2D) cultures, even in the presence of a stemness factor. We have previously shown that membrane translocation of the syntaxin4, t-SNARE protein contributes to this phenomenon. Here, we show that ES cells in three-dimensional (3D) aggregates do not succumb to extruded syntaxin4 owing to suppressed expression of P-cadherin protein. While extracellular expression of syntaxin4 led to the striking upregulation of P-cadherin mRNA in both 2D and 3D-ES cells, morphological changes and appreciable expression of P-cadherin protein were detected only in 2D-ES cells. Importantly, the introduction of an expression cassette for P-cadherin practically reproduced the effects induced by extracellular syntaxin4, where the transgene product was clearly detected in 2D-, but not 3D-ES cells. An expression construct for P-cadherin-Venus harboring an in-frame insertion of the P2A sequence at the joint region gave fluorescent signals only in the cytoplasm of 2D-ES cells, demonstrating translational regulation of P-cadherin. These results provide the mechanistic insight into the uncontrollable differentiation in 2D-ES cells and shed light on the validity of the “embryoid body protocol commonly used for ES cell handling” for directional differentiation. Key words: differentiation, embryoid body, ES cells, P-cadherin, syntaxin4 |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0386-7196 1347-3700 |
| Relation: | https://www.jstage.jst.go.jp/article/csf/48/1/48_22060/_html/-char/en; https://doaj.org/toc/0386-7196; https://doaj.org/toc/1347-3700 |
| DOI: | 10.1247/csf.22060 |
| URL الوصول: | https://doaj.org/article/e6bb222ef11e40ad86197631fac07144 |
| رقم الأكسشن: | edsdoj.6bb222ef11e40ad86197631fac07144 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 03867196 13473700 |
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| DOI: | 10.1247/csf.22060 |